%0 Journal Article %A Williamson, C. %A von Wechmar, M. B. %T Two novel viruses associated with severe disease symptoms of the green stinkbug Nezara viridula %D 1992 %J Journal of General Virology, %V 73 %N 9 %P 2467-2471 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-73-9-2467 %I Microbiology Society, %X Two viruses were isolated from green stinkbugs (Nezara viridula) with severe disease symptoms. These viruses have been named N. viridula virus type 1 (NVV-1) and NVV-2 according to their relative sedimentation coefficients. NVV-1 is a small picorna-like virus with a diameter of 29 nm, a buoyant density in CsCl of 1.34 g/ml and a sedimentation coefficient of 153S. NVV-1 particles contain a 9.4 kb ssRNA segment and have three coat proteins of M rs 32100, 31500 and 30700. NVV-2 sediments as two components on sucrose gradients; the top 104S component consists almost entirely of 41 nm empty capsids and the faster sedimenting 177S component consists of intact 39 nm spherical particles. NVV-2 particles have a buoyant density in CsCl of 1.39 g/ml and consist of one major protein of M r 73800 and at least two minor proteins of M rs 13500 and 16500. Only one dsRNA segment of 6.2 kb was identified. The properties of NVV-2 are similar to those of the Totiviridae. Individual stinkbugs were infected with either NVV-1 or NVV-2, or with a mixture of the two viruses. Re-infection of virus-free stinkbugs with the mixture resulted in typical disease symptoms. Both viruses were vertically transmitted through the eggs and insects were infected by surface contamination of their food source. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-73-9-2467