1887

Abstract

As a first step in the evaluation of the safety of replication-defective adenoviruses used as gene transfer vectors, the dissemination of wild-type (wt) adenovirus (Ad) type 5, Ad-dl327 (deleted for the E3 gene) and Ad- gp50 (deleted for E3 and E1A and therefore replication defective) was studied in mice and cotton rats. Of each virus, 10 p.f.u. was inoculated, either by the intranasal or the intramuscular route, and virus isolation was undertaken after sacrifice of the animals 3 or 31 days after inoculation. E3 deletion had no significant effect on viral spread, whereas El A deletion reduced it significantly. After intramuscular inoculation of the E3 — /E1A— virus, it could be isolated only from the local lymph node. Intranasal inoculation led to a wider distribution including lungs, liver, kidneys and lymph nodes. The pattern of dissemination of the E3 —/E1A— virus was the same in mice and cotton rats, providing indirect evidence of the lack of replication of this virus in this species permissive for the wt virus. However, in the case of infection with a wt strain in E3 —/E1A— adenovirus-inoculated recipients, both viruses were found in lymph nodes. In this situation the risk of phenotypic complementation of the E1A gene remains to be studied further.

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1994-10-01
2024-04-24
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References

  1. Adam M., Lepottier M. F., Eloit M. 1994; Vaccination of pigs with replication defective adenovirus: the example of pseudorabies. Veterinary Microbiology in press
    [Google Scholar]
  2. Andersson M., Paabo S., Nilsson T., Peterson P. A. 1985; Impaired intracellular transport of class I MHC antigens as a possible means for adenoviruses to evade immune surveillance. Cell 43:215–222
    [Google Scholar]
  3. Akli S., Caillaud C., Vigne E., Stratford-Perricaudet L. D., Poenaru L., Perricaudet M., Kahn A., Peschanski M. R. 1993; Transfer of a foreign gene into the brain using adenovirus vectors. Nature Genetics 3:224–228
    [Google Scholar]
  4. Betts A. O., Jennings A. R., Lamont P. H., Page Z. 1962; Inoculation of pigs with adenovirus of man. Nature; London: 19345–46
    [Google Scholar]
  5. Caillaud C., Akli S., Vigne E., Koulakoff A., Perricaudet M., Poenaru L., Kahn A., Berwald-Netter Y. 1993; Adenoviral vector as a gene delivery system into cultured rat neuronal and glial cells. European Journal of Neurosciences 5:1287–1291
    [Google Scholar]
  6. Cough R. B., Chanock R. M., Cate T. R., Lang D. J., Knight V., Huebner R. J. 1963; Immunization with types 4 and 7 adenovirus by selective infection of the intestinal tract. American Review of Respiratory Disease 88:394–403
    [Google Scholar]
  7. Eloit M., Gilardi-Hebestreit P., Toma B., Perricaudet M. 1990; Construction of a defective adenovirus vector expressing the pseudorabies virus glycoprotein gp50 and its use as a live vaccine. Journal of General Virology 71:2425–2431
    [Google Scholar]
  8. Ginsberg H. S., Lundholm-Beauchamp U., Horswood R. L., Pernis B., Wold W. S. M., Chanock R. M., Prince G. A. 1989; Role of early region 3 (E3) in pathogenesis of adenovirus disease. Proceedings of the National Academy of Sciences, U.S.A 86:3823–3827
    [Google Scholar]
  9. Ginsberg H. S., Moldawer L. L., Sehgal P. B., Redington M., Kilian P. L., Chanock R. M., Prince G. A. 1991; A mouse model for investigating the molecular pathogenesis of adenovirus pneumonia. Proceedings of the National Academy of Sciences, U.S.A 88:1651–1655
    [Google Scholar]
  10. Graham F. L., Smiley J., Russell W. C., Nairn R. 1977; Characteristics of a human cell line transformed by DNA from human adenovirus type 5. Journal of General Virology 36:59–72
    [Google Scholar]
  11. Guzman R. J., Lemarchand P., Crystal R. G., Epstein S. E., Finkel T. 1993; Efficient gene transfer into myocardium by direct injection of adenovirus vectors. Circulation Research 73:1202–1207
    [Google Scholar]
  12. Haddada H., Lopez M., Martinache C., Ragot T., Abina M. A., Perricaudet M. 1993; Efficient adenovirus-mediated gene transfer into human blood monocyte-derived macrophages. Biochemical and Biophysical Research Communications 195:1174–1183
    [Google Scholar]
  13. Jaffe H. A., Danel C., Longenecker G., Metzger M., Setoguchi Y., Rosenfeld M. A., Gant T. W., Thorgeirsson S. S., Stratford-Perricaudet L. D., Perricaudet M. 1992; Adenovirus-mediated in vivo gene transfer and expression in normal rat liver. Nature Genetics 1:372–378
    [Google Scholar]
  14. Jones N., Shenk T. 1978; Isolation of deletion and substitution mutants of adenovirus type 5. Cell 13:181–188
    [Google Scholar]
  15. Le Gal La Salle G., Robert J. J., Berrard S., Ridoux V., Stratford-Perricaudet L. D., Perricaudet M., Mallet J. 1993; An adenovirus vector for gene transfer into neurons and glia in the brain. Science 259:988–990
    [Google Scholar]
  16. Pacini D. L., Dubovi E. J., Clyde W. A. 1974; A new animal model for human respiratory tract disease due to adenovirus. Journal of Infectious Diseases 150:92–97
    [Google Scholar]
  17. Prevec L., Schneider M., Rosenthal K. L., Belbeck L. W., Derbyshire J. B., Graham F. L. 1989; Use of human adenovirus-based vectors for antigen expression in animals. Journal of General Virology 70:429–434
    [Google Scholar]
  18. Prince G. A., Porter D. D., Jenson A. B., Horswood R. L., Chanock R. M., Ginsberg H. S. 1993; Pathogenesis of adenovirus type 5 pneumonia in cotton rats (Sigmodon hispidus). Journal of Virology 67:101–111
    [Google Scholar]
  19. Ragot T., Finerty S., Watkins P. E., Perricaudet M., Morgan A. J. 1993a; Replication-defective recombinant adenovirus expressing the Epstein-Barr virus (EBV) envelope glycoprotein gp340/220 induces protective immunity against EBV-induced lymphomas in the cottontop tamarin. Journal of General Virology 74:501–507
    [Google Scholar]
  20. Ragot T., Vincent N., Chafey P., Vigne E., Gilgenkrantz H., Couton D., Cartaud J., Briand P., Kaplan J. C., Perricaudet M. 1993b; Efficient adenovirus-mediated transfer of a human minidystrophin gene to skeletal muscle of mdx mice. Nature; London: 361647–650
    [Google Scholar]
  21. Schneider M. D., French B. A. 1993; The advent of adenovirus-gene therapy for cardiovascular disease. Circulation 88:1937–1942
    [Google Scholar]
  22. Schwartz A. R., Togo Y., Hornick R. B. 1974; Clinical evaluation of live, oral types 1, 2 and 5 adenovirus vaccines. American Review of Respiratory Disease 109:233–238
    [Google Scholar]
  23. Stratford-Perricaudet L., Perricaudet M. 1991; Gene transfer into animals: the promise of adenovirus. In Human Gene Transfer pp. 51–61 Cohen-Haguenauer O., Boiron M. Edited by Montrouge: John Libbey Eurotext;
    [Google Scholar]
  24. Thimmapaya B., Jones N., Shenk T. 1982; Adenovirus VAI RNA is required for efficient translation of viral mRNAs at late times after infection. Cell 31:543–551
    [Google Scholar]
  25. Top F. H., Grossman R. A., Bartelloni P. J., Segal H. E., Dudding B. A., Russell P. K., Buscher E. L. 1971a; Immunization with live types 7 and 4 adenovirus vaccines. I. Safety, infectivity, antigenicity, and potency of adenovirus type 7 vaccine in humans. Journal of Infectious Diseases 124:148–154
    [Google Scholar]
  26. Top F. H., Buscher E. L., Bancroft W. H., Russell P. K. 1971b; Immunization with live types 7 and 4 adenovirus vaccines. II. Antibody response and protective effect against acute respiratory disease due to adenovirus type 7. Journal of Infectious Diseases 124:155–160
    [Google Scholar]
  27. Wesseling J. G., Godeke G.-J., Schijns V. E. C. J., Prevec L., Graham F. L., Horzinek M. C., Rottier P. J. M. 1993; Mouse hepatitis virus spike and nucleocapsid proteins expressed by adenovirus vectors protect mice against a lethal infection. Journal of General Virology 74:2061–2069
    [Google Scholar]
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