In vivo and in vitro trans-cleavage activity of hepatitis C virus serine proteinase expressed by recombinant baculoviruses Suzuki, Tetsuro and Sato, Mitsuru and Chieda, Shinya and Shoji, Ikuo and Harada, Takashi and Yamakawa, Yoshio and Watabe, Shoji and Matsuura, Yoshiharu and Miyamura, Tatsuo,, 76, 3021-3029 (1995), doi = https://doi.org/10.1099/0022-1317-76-12-3021, publicationName = Microbiology Society, issn = 0022-1317, abstract= By the use of recombinant baculoviruses, the trans-cleavage of hepatitis C virus (HCV) non-structural polyprotein was studied. The viral serine proteinase encoded by the NS3 gene was expressed efficiently in insect cells infected with a baculovirus recombined with HCV cDNA corresponding to amino acids 1046-1243 and the signal sequence of the rabies virus G protein. Coinfection studies showed the in vivo trans-cleavage activity of the expressed protein by the use of a recombinant producing NS5 as a substrate. We also found that the partially purified NS3 serine proteinase propared from the recombinant-infected cells could cleave NS5A/5B substrate. Characterization of the proteinase obtained will provide basic knowledge on processing of the HCV polyprotein., language=, type=