Classical swine fever virus-specific cytotoxic T lymphocytes and identification of a T cell epitope
Pauly, T. and Elbers, K. and König, M. and Lengsfeld, T. and Saalmüller, A. and Thiel, H.-J.,, 76, 3039-3049 (1995),
doi = https://doi.org/10.1099/0022-1317-76-12-3039,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= Classical swine fever virus (CSFV) -specific cytotoxic T lymphocytes (CTL) were derived from peripheral blood mononuclear leukocytes of immunized NIH-minipigs (MHC d/d haplotype) after in vitro restimulation with infectious CSFV. Their cytotoxic activity was determined against CSFV-infected target cells obtained from simian virus 40 (SV40) large T antigen-transfected immortalized kidney cells of a syngeneic miniature swine. Experiments with separated effector cell populations revealed that the CSFV-specific cytotoxic activity was mediated by CD4−CD6+CD8+ MHC class I-restricted T lymphocytes. Infection of target cells with various vaccinia virus/CSFV recombinants led to the identification of a major antigenic site for CSFV-specific CTL near the cleavage site between the non-structural proteins p80 (NS3) and p10 (NS4a). Using synthetic overlapping nonapeptides which covered this protein region the sequence ENALLVALF is the first sequence to be identified as an MHC class I-restricted T cell epitope recognized by CSFV-specific CTL.,
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