The detection of latency-associated transcripts of equine herpesvirus 1 in ganglionic neurons
Baxi, M. K. and Efstathiou, S. and Lawrence, G. and Whalley, J. M. and Slater, J. D. and Field, H. J.,, 76, 3113-3118 (1995),
doi = https://doi.org/10.1099/0022-1317-76-12-3113,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= Neural tissues from specific pathogen-free ponies that had been experimentally infected with equine herpesvirus 1 (EHV-1) were analysed by in situ hybridization. Digoxigenin-labelled EHV-1 BamHI fragments spanning almost the entire EHV-1 genome were hybridized to RNA in tissue sections from latently infected trigeminal ganglia. The BamHI E fragment detected EHV-1 RNA antisense to gene 63 (HSV-1 homologue ICP0) in a small number of neurons. Sixteen other BamHI fragments gave negative results in 20 sections tested with each fragment. Latency associated transcripts (LATs) were localized to the neuronal nuclei. EHV-1 nucleotide sequence data in the region reveals the presence of a putative EHV-1 LAT promoter that shares similar motifs with the HSV-1 LAT promoter, including the LAT promoter-binding factor, and may have a role in EHV-1 LAT expression.,
language=,
type=