1887

Abstract

Several allelic variants of the sheep PrP gene are associated with scrapie susceptibility. However, it is not known whether, and to what extent, the PrP genotype contributes to determining survival times of scrapie sheep. We therefore determined the PrP genotype and life spans of over 50 Flemish and Swifter sheep within a single scrapie-affected flock. Eighty-three per cent of the scrapie sheep were homozygous for the PrP allele (polymorphic amino acids at codons 136 and 171 are indicated) and these sheep died from scrapie at a mean age of 25 months. In sheep heterozygous for PrP, development of scrapie was delayed or did not occur. Sheep with at least one PrP allele, including PrP/PrP sheep, did not develop scrapie. No scrapie sheep were found without a PrP allele. We conclude that the PrP genotype contributes to determining survival times of sheep with natural scrapie. Additionally, we describe two novel sheep PrP allelic variants.

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1996-10-01
2024-04-20
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References

  1. Belt P. B. G. M., Muileman I. H., Schreuder B. E. C., Bos-de Ruijter J., Gielkens A. L. J., Smits M. A. 1995; Identification of five allelic variants of the sheep PrP gene and their association with natural scrapie. Journal of General Virology 76:509–517
    [Google Scholar]
  2. Belt P. B. G. M., Bossers A., Schreuder B. E. C., Smits M. A. 1996; PrP allelic variants associated with natural scrapie. In Bovine Spongiform Encephalopathy; The BSE Dilemma Edited by Gibbs Clarence J. Jr New York: Springer;
    [Google Scholar]
  3. Clouscard C., Beaudry P., Elsen J. M., Milan D., Dussaucy M., Bounneau C., Schelder F., Chatelain J., Launay J. M., Laplanche J. L. 1995; Different allelic effects of the codons 136 and 171 of the prion protein gene in sheep with natural scrapie. Journal of General Virology 76:2097–2101
    [Google Scholar]
  4. Dickinson A. G., Young G. B., Stamp J. T., Renwick C. C. 1965; An analysis of natural scrapie in Suffolk sheep. Heredity 20:485–503
    [Google Scholar]
  5. Dickinson A. G., Stamp J. T., Renwick C. C. 1974; Maternal and lateral transmission of scrapie in sheep. Journal of Comparative Pathology 84:19–25
    [Google Scholar]
  6. Foster J. D., Dickinson A. G. 1988; The unusual properties of CH1641, a sheep-passaged isolate of scrapie. Veterinary Record 123:5–8
    [Google Scholar]
  7. Goldmann W., Hunter N., Foster J. D., Salbaum J. M., Beyreuther K., Hope J. 1990; Two alleles of a neural protein gene linked to scrapie in sheep. Proceedings of the National Academy of Sciences, USA 87:2476–2480
    [Google Scholar]
  8. Goldmann W., Hunter N., Benson G., Foster J. D., Hope J. 1991; Different scrapie-associated fibril proteins (PrP) are encoded by lines of sheep selected for different alleles of the Sip gene. Journal of General Virology 72:2411–2417
    [Google Scholar]
  9. Goldmann W., Hunter N., Smith G., Foster J., Hope J. 1994; PrP genotype and agent effects in scrapie: change in allelic interaction with different isolates of agent in sheep, a natural host of scrapie. Journal of General Virology 75:989–995
    [Google Scholar]
  10. Hunter N., Goldmann W., Benson G., Foster J. D., Hope J. 1993; Swaledale sheep affected by natural scrapie differ significantly in PrP genotype frequencies from healthy sheep and those selected for reduced incidence of scrapie. Journal of General Virology 74:1025–1031
    [Google Scholar]
  11. Hunter N., Goldmann W., Smith G., Hope J. 1994; The association of a codon 136 PrP gene variant with the occurrence of natural scrapie. Archives of Virology 137:171–177
    [Google Scholar]
  12. Hunter N., Foster J. D., Goldmann W., Hope J., Bostock C. 1995; Natural scrapie in a closed flock of Cheviot sheep affects animals encoding specific PrP protein variants. International Symposium on prion diseases Gӧttingen, Germany, abstract 69
    [Google Scholar]
  13. Ikeda T., Horiuchi M., Ishiguro N., Muramatsu Y., Kai-uwe G. D., Shinagawa M. 1995; Amino acid polymorphisms of PrP with reference to onset of scrapie in Suffolk and Corriedale sheep in Japan. Journal of General Virology 76:2577–2581
    [Google Scholar]
  14. Laplanche J. L., Chatelain J., Westaway D., Thomas S., Dussaucy M., Brugere-Picoux J., Launay J. M. 1993; PrP polymorphisms associated with natural scrapie discovered by denaturing gradient gel electrophoresis. Genomics 15:30–37
    [Google Scholar]
  15. Maciulis A., Hunter N., Wang S., Goldmann W., Hope J., Foote W. 1992; Polymorphisms of a scrapie-associated fibril protein (PrP) gene and their association with susceptibility to experimentally induced scrapie in Cheviot sheep in the United States. American Journal of Veterinary Research 53:1957–1960
    [Google Scholar]
  16. Prusiner S. B. 1982; Novel proteinaceous infection particles cause scrapie. Science 216:136–144
    [Google Scholar]
  17. Prusiner S. B. 1991; Molecular biology of prion diseases. Science 252:1515–1522
    [Google Scholar]
  18. Prusiner S. B. 1993; Genetic and infectious prion’s diseases. Archives in Neurology 50:1129–1153
    [Google Scholar]
  19. Walsh P. S., Metger D. A., Higuchi R. 1991; Chelex 100’ as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10:506–513
    [Google Scholar]
  20. Westaway D., Zuliani V., Cooper C. M., Dacosta M., Neuman S., Jenny A. L., Detwiler L., Prusiner S. B. 1994; Homozygosity for prion protein alleles encoding glutamine-171 renders sheep susceptible to natural scrapie. Genes & Development 8:959–969
    [Google Scholar]
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