1887

Abstract

A new viroid associated with an apple fruit disorder similar to, but more severe than, the dapple apple disease induced in some varieties by apple scar skin viroid (ASSVd) has been found. The new viroid, tentatively termed apple dimple fruit viroid (ADFVd), is a circular RNA of 306 nucleotides which adopts a quasi-rod-like conformation of minimum free energy. It contains the core nucleotides of the central conserved region (CCR) of the ASSVd group as well as the terminal conserved region (TCR) present in all members of the ASSVd and potato spindle tuber viroid (PSTVd) monophyletic groups. ADFVd has the highest sequence similarity with ASSVd and the 294 nucleotide citrus viroid CVd-IIIb, sharing with the latter an almost identical left terminal domain. The right- and left-hand termini of ADFVd are formed by almost perfect duplications of sequences found in the CCR upper and lower strands, respectively, of PSTVd and closely related viroids.

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1996-11-01
2024-04-25
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References

  1. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  2. Diener T. O. 1986; Viroid processing: a model involving the central conserved region of hairpin I. Proceedings of the National Academy of Sciences, USA 83:58–62
    [Google Scholar]
  3. Diener T. O. 1991; Sub viral pathogens of plants: viroids and viroidlike satellite RNAs. FASEB Journal 5:2808–2813
    [Google Scholar]
  4. Flores R. 1986; Detection of citrus exocortis viroid in crude extracts by dot-blot hybridization: conditions for reducing spurious hybridization results and for enhancing the sensitivity of the technique. Journal of Virological Methods 13:161–169
    [Google Scholar]
  5. Flores R. 1995; Viroids. In Virus Taxonomy. Sixth Report of the International Committee on Taxonomy of Viruses pp 495–497 Edited by Murphy F. A., Fauquet C. M., Bishop D. H. L., Ghabrial S. A., Jarvis A. W., Martelli G.P., Mayo M. A., Summers M. D. Wien & New York: Springer Verlag;
    [Google Scholar]
  6. Flores R., Durán-Vila N., Pallás V., Semancik J. S. 1985; Detection of viroid and viroid-like RNAs from grapevine. Journal of General Virology 66:2095–2102
    [Google Scholar]
  7. Hashimoto J., Koganezawa H. 1987; Nucleotide sequence and secondary structure of apple scar skin viroid. Nucleic Acids Research 15:7045–7052
    [Google Scholar]
  8. Hernández C., Elena S. F., Moya A., Flores R. 1992; Pear blister canker viroid is a member of the apple scar skin subgroup (apscaviroids) and also has sequence homology with viroids from other subgroups. Journal of General Virology 73:2503–2507
    [Google Scholar]
  9. Ito T., Kanematsu S., Koganezawa H., Tsuchizaki T., Yoshida K. 1993; Detection of a viroid associated with apple fruit crinkle disease. Annals of the Phytopathological Society of Japan 59:520–527
    [Google Scholar]
  10. Koganezawa H. 1989; Apple scar skin viroid. AAB Descriptions of Plant Viruses number 349
    [Google Scholar]
  11. Koltunow A. M., Rezaian M. A. 1988; Grapevine yellow speckle viroid. Structural features of a new viroid group. Nucleic Acids Research 16:849–864
    [Google Scholar]
  12. Koltunow A. M., Rezaian M. A. 1989; A scheme for viroid classification. Intervirology 30:194–201
    [Google Scholar]
  13. Navarro B., Daròs J. A., Flores R. 1996; A general strategy for cloning viroids and other small circular RNAs that uses minimal amounts of template and does not require prior knowledge of its sequence. Journal of Virological Methods 56:59–66
    [Google Scholar]
  14. Pallás V., Navarro A., Flores R. 1987; Isolation of a viroid-like RNA from hop different from hop stunt viroid. Journal of General Virology 68:3201–3205
    [Google Scholar]
  15. Puchta H., Luckinger R., Yang X., Hadidi A., Sanger H. L. 1990; Nucleotide sequence and secondary structure of apple scar skin viroid (ASSVd) from China. Plant Molecular Biology 14:1065–1067
    [Google Scholar]
  16. Rakowski A. G., Szychowski J. A., Avena Z. S., Semancik J. S. 1994; Nucleotide sequence and structural features of the Group III citrus viroids. Journal of General Virology 75:3581–3584
    [Google Scholar]
  17. Sänger H. L., Ramm K., Domdey H., Gross H. J., Henco K., Riesner D. 1977; Conversion of circular viroid molecules to linear strands. FEES Letters 99:117–122
    [Google Scholar]
  18. Stasys R. A., Dry I. A., Rezaian M. A. 1995; The termini of a new citrus viroid contain duplications of the central conserved region of two viroid groups. FEBS Letters 358:182–184
    [Google Scholar]
  19. Visvader J. E., Forster A. C., Symons R. H. 1985; Infectivity and in vitro mutagenesis of monomeric cDNA clones of citrus exocortis viroid indicate the site of processing of viroid precursors. Nucleic Acids Research 13:5843–5856
    [Google Scholar]
  20. Zuker M. 1989; On finding all suboptimal foldings of an RNA molecule. Science 244:48–52
    [Google Scholar]
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