RT Journal Article SR Electronic(1) A1 Vapalahti, Olli A1 Lundkvist, Åke A1 Kukkonen, Sami K. J. A1 Cheng, Ying A1 Gilljam, Mari A1 Kanerva, Mari A1 Manni, Tytti A1 Pejcoch, Milan A1 Niemimaa, Jukka A1 Kaikusalo, Asko A1 Henttonen, Heikki A1 Vaheri, Antti A1 Plyusnin, AlexanderYR 1996 T1 Isolation and characterization of Tula virus, a distinct serotype in the genus Hantavirus, family Bunyaviridae JF Journal of General Virology, VO 77 IS 12 SP 3063 OP 3067 DO https://doi.org/10.1099/0022-1317-77-12-3063 PB Microbiology Society, SN 1465-2099, AB A Vero E6 cell culture isolate of Tula virus (TUL), a hantavirus first detected in European common voles (Microtus arvalis and M. rossiaemeridionalis) by RT-PCR was obtained after initial passaging of TUL-infected vole lung samples in laboratory-colonized M. arvalis. TUL was defined as a classical serotype by a cross-focus-reduction neutralization test (FRNT) and was also shown to be distinct from other hantaviruses by haemagglutination inhibition assay. The sequences of S, M and partial L genome segments of the isolate were determined: the S segment was 99.9% identical to the original rodent-derived sequence. Serological evidence for a previous TUL infection was obtained from the serum of a blood donor living near a TUL focus in Moravia, Czech Republic, showing at least a 16-fold higher FRNT titre to TUL as compared to Puumala or other hantaviruses., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-77-12-3063