1887

Abstract

The interaction of influenza virus NS1 protein with other viral products in the infected cell was analysed by co-immunoprecipitation studies. The three subunits of the polymerase and the nucleoprotein, but not M1 protein, were co-immunoprecipitated by NS1-specific serum but not when control serum was used. Such co-immunoprecipitation was not sensitive to RNase treatment of the immuno- precipitates. Co-immunoprecipitation was also obtained when the viral transcription-replication system was reconstituted by transfection of cDNAs and model vRNA template into vaccinia virus-T7-infected cells. Analysis of the RNA pulled- down in the NSI-specific precipitates indicated the presence of both vRNA and mRNA. These results are discussed in the context of the phenotype of virus temperature-sensitive mutants affected in the NS1 gene.

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1997-10-01
2024-04-19
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References

  1. Chien C.-T., Bartel R., Sternglanz R., Fields S. 1991; The two- hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest. Proceedings of the National Academy of Sciences USA: 889578–9582
    [Google Scholar]
  2. de la Luna S., Fortes P., Beloso A., Ortín J. 1995; Influenza virus NS1 protein enhances the rate of translation initiation of viral mRNAs. Journal of Virology 69:2427–2433
    [Google Scholar]
  3. Enami K., Sato T. A., Nakada S., Enami M. 1994; Influenza virus NS1 protein stimulates translation of the M1 protein. Journal of Virology 68:1432–1437
    [Google Scholar]
  4. Fortes P., Beloso A., Ortín J. 1994; Influenza virus NS1 protein inhibits pre-mRNA splicing and blocks RNA nucleocytoplasmic trans-port. EMBO Journal 13:704–712
    [Google Scholar]
  5. Fortes P., Lamond A. I., Ortín J. 1995; Influenza virus NS1 protein alters the subnuclear localization of cellular splicing components. Journal of General Virology 76:1001–1007
    [Google Scholar]
  6. Gonzalez S., Zürcher T., Ortín J. 1996; Identification of two separate domains in the influenza virus PB1 protein responsible for interaction with the PB2 and PA subunits: a model for the viral RNA polymerase structure. Nucleic Acids Research 24:4456–4463
    [Google Scholar]
  7. Hatada E., Hasegawa M., Shimizu K., Hatanaka M., Fukuda R. 1990; Analysis of influenza A virus temperature-sensitive mutants with mutations in RNA segment 8. Journal of General Virology 71:1283–1292
    [Google Scholar]
  8. Hatada E., Saito S., Okishio N., Fukuda R. 1997; Binding of the influenza virus NS1 protein to model genome RNAs. Journal of General Virology 78:1059–1063
    [Google Scholar]
  9. Hay A. J. 1982; Characterization of influenza virus RNA complete transcripts. Virology 116:517–522
    [Google Scholar]
  10. Honda A., Mukaigawa J., Yokoiyama A., Kato A., Ueda S., Nagata K., Krystal M., Nayak D. P., Ishihama A. 1990; Purifcation and molecular structure of RNA polymerase from influenza virus A/PR8. Journal of Biochemistry 107:624–628
    [Google Scholar]
  11. Kato A., Mizumoto K., Ishihama A. 1985; Purification and enzymatic properties of an RNA polymerase-RNA complex from influenza virus. Virus Research 3:115–127
    [Google Scholar]
  12. Koennecke I., Boschek C. B., Scholtissek C. 1981; Isolation and properties of a temperature-sensitive mutant (ts412) of the influenza virus recombinant with a lesion in the gene coding for the nonstructural protein. Virology 110:16–25
    [Google Scholar]
  13. Krug R. M., Alonso-Kaplen F. V., Julkunen I., Katze M. G. 1989; Expression and replication of the influenza virus genome. In The Influenza Viruses pp 89–152 Krug R. M. Edited by New York: Plenum Press;
    [Google Scholar]
  14. Lamb R. A. 1989; The genes and proteins of influenza viruses. In The Influenza Viruses pp 1–87 Krug R. M. Edited by New York: Plenum Press;
    [Google Scholar]
  15. Ludwig S., Vogel U., Scholtissek C. 1995; Amino acid replacements leading to temperature-sensitive defects of the NS1 protein of influenza A virus. Archives of Virology 140:945–950
    [Google Scholar]
  16. Mena I., de la Luna S., Albo C., Martín J., Nieto A., Ortín J., Portela A. 1994; Synthesis of biologically active influenza virus core proteins using a vaccinia virus-T7 RNA polymerase expression system. Journal of General Virology 75:2109–2114
    [Google Scholar]
  17. Nieto A., delaLuna S., Bárcena J., Portela A., Valcárcel J., Melero J. A., Ortín J. 1992; Nuclear transport of influenza virus polymerase PA protein. Virus Research 24:65–75
    [Google Scholar]
  18. Park Y. W., Katze M. G. 1995; Translational control by influenza virus. Identification of cis-acting sequences and trans-acting factors which may regulate selective viral mRNA translation. Journal of Biological Chemistry 270:28433–28439
    [Google Scholar]
  19. Perales B., Ortín J. 1997; The influenza A virus PB2 polymerase subunit is required for the replication of viral RNA. Journal of Virology 71:1381–1385
    [Google Scholar]
  20. Qiu Y., Krug R. M. 1994; The influenza virus NS1 protein is a poly(A)-binding protein that inhibits nuclear export of mRNAs containing poly(A). Journal of Virology 68:2425–2432
    [Google Scholar]
  21. Qiu Y., Nemeroff M., Krug R. M. 1995; The influenza virus NS1 protein binds to a specific region in human U6 snRNA and inhibits U6-U2 and U6-U4 snRNA interactions during splicing. RNA 1:304–316
    [Google Scholar]
  22. Richardson J. C., Akkina R. K. 1991; NS2 protein of influenza virus is found in purified virus and phosphorylated in infected cells. Archives of Virology 116:69–80
    [Google Scholar]
  23. Scholtissek C., Spring S. 1982; Extragenic suppression of temperature-sensitive mutations in RNA segment 8 by replacement of different RNA segments with those of other influenza A virus prototype strains. Virology 118:28–34
    [Google Scholar]
  24. Tiley L. S., Hagen M., Mathews J. T., Krystal M. 1994; Sequence- specific binding of the influenza virus RNA polymerase to sequences locatedat the 5ʹ-end of the viral RNAs. Journal of Virology 68:5108–5116
    [Google Scholar]
  25. Zürcher T., de la Luna S., Sanz-Ezquerro J. J., Nieto A., Ortín J. 1996; Mutational analysis of the influenza virus A/Victoria/3/75 PA protein: studies of interaction with PB1 protein and identification of a dominant negative mutant. Journal of General Virology 77:1745–1749
    [Google Scholar]
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