@article{mbs:/content/journal/jgv/10.1099/0022-1317-78-11-2789, author = "Heusipp, Gerhard and Grötzinger, Carsten and Herold, Jens and Siddell, Stuart G and Ziebuhr, John", title = "Identification and subcellular localization of a 41 kDa, polyprotein 1ab processing product in human coronavirus 229E-infected cells.", journal= "Journal of General Virology", year = "1997", volume = "78", number = "11", pages = "2789-2794", doi = "https://doi.org/10.1099/0022-1317-78-11-2789", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-78-11-2789", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "The translation products of the human coronavirus (HCV) 229E open reading frames 1a and 1b, the polyproteins 1a and 1ab, are processed by virus- encoded proteinases. One of the key enzymes in this process is a chymotrypsin-like enzyme, the 3C- like proteinase. In this study we have identified an ORF 1b-encoded, 41 kDa processing product in HCV 229E-infected cells by using a monoclonal antibody with defined specificity. We show that this polypeptide is released from polyprotein 1ab by 3C-like proteinase-mediated cleavage of the peptide bonds Gln-6110/Gly-6111 and Gln-6458/Ser- 6459. Also, we have investigated the subcellular localization of the 41 kDa processing product. Immunofluorescence microscopy revealed a punctate, perinuclear distribution of the 41 kDa polypeptide in infected cells and an identical subcellular localization was observed for three additional pp1ab-derived polypeptides. In contrast, the virus nucleocapsid protein showed a homogeneous cytoplasmic localization.", }