1887

Abstract

In order to evaluate genetic variation between rabbit haemorrhagic disease virus (RHDV) isolates and to derive phylogenetic relationships, 56 virus isolates collected from various parts of France over a 7 year period (1988 to 1995) were examined. Analyses were carried out by direct nucleotide sequencing of PCR fragments of three genomic regions encoding the capsid protein (VP60) (regions A and B) and a non-structural protein (region C). Multiple sequence alignments revealed maximum nucleotide divergence of 7·6, 9·4 and 8·7% for regions A, B and C, respectively, indicating a high level of conservation between isolates. Irrespective of the genomic region analysed, phylogenetic analyses carried out using various methods allowed the identification of three genogroups; distribution of isolates within these genogroups appears to be more related to the year of their collection than to their geographical origin. The possible evolution of RHDV is discussed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-79-1-11
1998-01-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/79/1/9460916.html?itemId=/content/journal/jgv/10.1099/0022-1317-79-1-11&mimeType=html&fmt=ahah

References

  1. Bouslama A., De Mia G. M., Hammami S., Aouina T., Soussi H., Frescura T. 1996; Identification of the virus of rabbit haemorrhagic disease in Tunisia. Veterinary Research 138:108–110
    [Google Scholar]
  2. Capucci L., Frigoli G., Ronshold L., Lavazza A., Brocchi E., Rossi C. 1995; Antigenicity of the rabbit hemorrhagic disease virus studied by its reactivity with monoclonal antibodies. Virus Research 37:221–238
    [Google Scholar]
  3. Capucci L., Fusi P., Lavazza A., Pacciarini M. L., Rossi C. 1996; Detection and preliminary characterization of a new rabbit calicivirus related to rabbit hemorrhagic disease virus but non-pathogenic. Journal of Virology 70:8614–8623
    [Google Scholar]
  4. Chasey D., Trout R. C. 1995; Rabbit haemorrhagic disease in Britain. Mammalia 59:599–603
    [Google Scholar]
  5. Collery P. M., Mooney J., O’Connor M. 1995; Rabbit haemorrhagic disease in Ireland. Veterinary Record 137:547
    [Google Scholar]
  6. Felsenstein J. 1993; PHYLIP: Phylogeny interference package. version 3.5 University of Washington, Seattle, Wa., USA:
    [Google Scholar]
  7. Gregg D. A., House C. 1989; Necrotic hepatitis of rabbits in Mexico: a parvovirus. Veterinary Record 125:603–604
    [Google Scholar]
  8. Le Gall G., Huguet S., Vende P., Vautherot J. F., Rasschaert D. 1996; European brown hare syndrome virus: molecular cloning and sequencing of the genome. Journal of General Virology 77:1693–1697
    [Google Scholar]
  9. Liu S. J., Xue H. P., Pu B. Q., Qian N. 1984; A new viral disease in rabbits. Animal Husbandry and Veterinary Medicine 16:253–255
    [Google Scholar]
  10. Meyers G., Wirblich C., Thiel H. J. 1991; Genomic and subgenomic RNAs of rabbit hemorrhagic disease virus are both protein-linked and packaged into particles. Virology 184:677–686
    [Google Scholar]
  11. Morisse J. P., Le Gall G., Boilletot E. 1991; Hepatitis of viral origin in ntroduction and aetiological hypotheses. Revue Scientifique et Technique Office International des Epizooties 10:371–392
    [Google Scholar]
  12. Neill J. D. 1992; Nucleotide sequence of the capsid protein gene of two serotypes of San Miguel sea lion virus: identification of conserved and non-conserved amino acid sequences among caliciviruses capsid proteins. Virus Research 24:211–222
    [Google Scholar]
  13. Nowotny N., Ros Bascunana C., Ballagi-Pordany A., Gavier-Widen D., Uhlén M., Belak S. 1997; Phylogenetic analysis of rabbit haemorrhagic disease and European brown hare syndrome viruses by comparison of sequences from the capsid protein gene. Archives of Virology 142:657–673
    [Google Scholar]
  14. Patterson I. A. P., Howie F. E. 1995; Rabbit haemorrhagic disease in Scotland. Veterinary Record 137:523
    [Google Scholar]
  15. Rasschaert D., Huguet S., Madeleine M. F., Vautherot J. F. 1994; Sequence and genomic organization of a rabbit hemorrhagic disease virus isolated from a wild rabbit. Virus Genes 9:121–132
    [Google Scholar]
  16. Vende P., Le Gall G., Rasschaert D. 1995; An alternative method for direct sequencing of PCR products for epidemiological studies performed by nucleic sequence comparison.Application to rabbit haemorrhagic disease virus. Veterinary Research 26:174–179
    [Google Scholar]
  17. Wirblich C., Thiel H. J., Meyers G. 1996; Genetic map of the calicivirus rabbit hemorrhagic disease virus as deduced from in vitro translation studies. Journal of Virology 70:7974–7983
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-79-1-11
Loading
/content/journal/jgv/10.1099/0022-1317-79-1-11
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error