1887

Abstract

HPRS-103, the prototype of avian leukosis virus (ALV) subgroup J, is a recently identified retrovirus associated with myeloid leukosis in meat-type chickens. Although this virus shows high sequence identity to other ALV subgroups within the gag and pol genes, its env gene is highly diverged (with only about 40% sequence identity) from other ALV subgroups. On the other hand, the sequence of the env gene of HPRS-103 was 75% identical to that of E51, a member of the EAV family of endogenous avian retroviruses. It is reported here that the chicken genome also contains another EAV-related element, EAV-HP, showing much greater sequence identity (over 97%) to the HPRS-103 env gene. Southern blotting analysis showed that EAV-HP-related sequences were distinct from EAV-O and were present in all lines of chicken examined and in grey jungle fowl, but were absent from several other avian species. The potential role of these endogenous sequences in the evolution of ALV subgroup J viruses is discussed.

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1999-01-01
2024-03-28
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References

  1. Astrin S. M., Buss E. G., Hayward W. S. 1979; Endogenous viral genes are non-essential in the chicken. Nature 282:339–341
    [Google Scholar]
  2. Bai J., Payne L. N., Skinner M. A. 1995; HPRS-103 (exogenous avian leukosis virus subgroup J) has an env gene related to those of endogenous elements EAV-0 and E51 and an E element found previously only in sarcoma viruses. Journal of Virology 76:181–187
    [Google Scholar]
  3. Benkel B. F., Gavora J. S. 1993; A novel molecular fingerprint probe based on the endogenous avian retroviral element (EAV) of chickens. Animal Genetics 24:409–413
    [Google Scholar]
  4. Boyce-Jacino M. T., Resnick R., Faras A. J. 1989; Structural and functional characterization of the unusually short long terminal repeats and their adjacent regions of a novel endogenous avian retrovirus. Virology 173:157–166
    [Google Scholar]
  5. Boyce-Jacino M. T., O’Donoghue K., Faras A. J. 1992; Multiple complex families of endogenous retroviruses are highly conserved in the genus Gallus. Journal of Virology 66:4919–4929
    [Google Scholar]
  6. Bumstead N., Palyga J. 1992; A preliminary linkage map of the chicken genome. Genomics 13:690–697
    [Google Scholar]
  7. Chakrabarti R., Hofman F. M., Pandey R., Mathes L. E., Roy-Burman P. 1994; Recombination between feline exogenous and endogenous retroviral sequences generates tropism for cerebral endothelial cells. American Journal of Pathology 144:348–358
    [Google Scholar]
  8. Crittenden L. B. 1991; Retroviral elements in the genome of the chickens: implications for poultry genetics and breeding. Critical Reviews in Poultry Biology 3:73–109
    [Google Scholar]
  9. Crittenden L. B., Salter D. W. 1998; Spontaneous mobility of subgroup A avian leukosis virus proviruses in the genome of the chicken. Avian Pathology 27:S26–S35
    [Google Scholar]
  10. Dunwiddie C., Faras A. J. 1985; Presence of retrovirus reverse transcriptase gene sequences in avian cells lacking endogenous avian leukosis viruses. Proceedings of the National Academy of Sciences, USA 82:5097–5101
    [Google Scholar]
  11. Hughes S. H., Mutschler A., Bishop J. M., Varmus H. E. 1981; Rous sarcoma virus provirus is flanked by short direct repeats of a cellular DNA sequence present in only one copy prior to integration. Proceedings of the National Academy of Sciences, USA 78:4299–4305
    [Google Scholar]
  12. Manley K. F. 1993; A Macintosh program for storage and analysis of experimental genetic mapping data. Mammalian Genome 4:303–313
    [Google Scholar]
  13. Payne L. N., Brown S. R., Bumstead N., Howes K., Frazier J. A., Thouless M. E. 1991; A novel subgroup of exogenous avian leukosis virus in chickens. Journal of General Virology 72:801–807
    [Google Scholar]
  14. Payne L. N., Howes K., Gillespie A. M., Smith L. M. 1992; Host range of Rous sarcoma virus pseudotype RSV(HPRS-103) in 12 avian species: support for a new avian retrovirus envelope subgroup, designated J. Journal of General Virology 73:2995–2997
    [Google Scholar]
  15. Resnick R. M., Boyce-Jacino M. T., Fu Q., Faras A. J. 1990; Phylogenetic distribution of the novel avian endogenous provirus family EAV-0. Journal of Virology 64:4640–4653
    [Google Scholar]
  16. Robertson J. S., Nicolson C., Riley A.-M., Bentley M., Dunn G., Corcoran T., Schild G. C., Minor P. 1997; Assessing the significance of reverse transcriptase activity in chick cell-derived vaccines. Biologicals 25:403–414
    [Google Scholar]
  17. Rovigati U. G., Astrin S. M. 1983; Avian endogenous viral genes. Current Topics in Microbiology and Immunology 103:1–21
    [Google Scholar]
  18. Roy-Burman P. 1995; Endogenous env elements: partners in generation of pathogenic feline leukaemia viruses. Virus Genes 11:147–161
    [Google Scholar]
  19. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Biology. A Laboratory Manual 2nd edn Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  20. Smith L. M., Brown S. R., Howes K., McLeod S., Arshad S. S., Barron G. S., Venugopal K., McKay J. C., Payne L. N. 1998; Development and application of PCR tests for the detection of subgroup J avian leukosis virus. Virus Research 54:87–98
    [Google Scholar]
  21. Stoye J. P., Coffin J. M. 1987; The four classes of endogenous murine leukemia viruses: structural relationships and potential for recombination. Journal of Virology 61:2659–2669
    [Google Scholar]
  22. Toye A. A., Schalkwyk L., Lehrach H., Bumstead N. 1997; A yeast artificial chromosome (YAC) library containing 10 haploid chicken genome equivalents. Mammalian Genome 8:274–276
    [Google Scholar]
  23. Venugopal K., Smith L. M., Howes K., Payne L. N. 1998; Antigenic variants of J subgroup avian leukosis virus: sequence analysis reveals multiple changes in the env gene. Journal of General Virology 79:757–766
    [Google Scholar]
  24. Weissmahr R. N., Schupbach J., Boni J. 1997; Reverse transcriptase activity in chicken embryo fibroblast culture supernatants is associated with particles containing endogenous avian retrovirus EAV-0 RNA. Journal of Virology 71:3005–3012
    [Google Scholar]
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