%0 Journal Article %A Liang, Guo-Dong %A Li, Lei %A Zhou, Guo-Lin %A Fu, Shi-Hong %A Li, Qi-Ping %A Li, Fu-Sheng %A He, Hai-Huai %A Jin, Qi %A He, Ying %A Chen, Bo-Quan %A Hou, Yun-De %T Isolation and complete nucleotide sequence of a Chinese Sindbis-like virus %D 2000 %J Journal of General Virology, %V 81 %N 5 %P 1347-1351 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-81-5-1347 %I Microbiology Society, %X Infection with alphaviruses is common in the Chinese population. Here we report the isolation of a Sindbis-like virus from a pool of Anopheles mosquitoes collected in Xinjiang, China during an arbovirus survey. This virus, designated XJ-160, rapidly produced cytopathic effects on mosquito and hamster cells. In addition, it was lethal to neonatal mice if inoculated intracerebrally. Serologically, XJ-160 reacted with and was neutralized by an anti-Sindbis antibody. Anti-XJ-160 antibodies were found in several cohorts of Chinese subjects. The complete 11626-base nucleotide sequence of XJ-160 was determined. XJ-160 has diverged significantly from the prototype Sindbis virus, with an 18% difference in nucleotide sequence and an 8ยท6% difference in amino acids; there are 11 deletions and 2 insertions, involving 99 nucleotides in total. XJ-160 is most closely linked to Kyzylagach virus isolated in Azerbaijan. Both belong to the African/European genetic lineage of Sindbis virus, albeit more distantly related to other members. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-81-5-1347