RT Journal Article SR Electronic(1) A1 Liang, Guo-Dong A1 Li, Lei A1 Zhou, Guo-Lin A1 Fu, Shi-Hong A1 Li, Qi-Ping A1 Li, Fu-Sheng A1 He, Hai-Huai A1 Jin, Qi A1 He, Ying A1 Chen, Bo-Quan A1 Hou, Yun-De YR 2000 T1 Isolation and complete nucleotide sequence of a Chinese Sindbis-like virus JF Journal of General Virology, VO 81 IS 5 SP 1347 OP 1351 DO https://doi.org/10.1099/0022-1317-81-5-1347 PB Microbiology Society, SN 1465-2099, AB Infection with alphaviruses is common in the Chinese population. Here we report the isolation of a Sindbis-like virus from a pool of Anopheles mosquitoes collected in Xinjiang, China during an arbovirus survey. This virus, designated XJ-160, rapidly produced cytopathic effects on mosquito and hamster cells. In addition, it was lethal to neonatal mice if inoculated intracerebrally. Serologically, XJ-160 reacted with and was neutralized by an anti-Sindbis antibody. Anti-XJ-160 antibodies were found in several cohorts of Chinese subjects. The complete 11626-base nucleotide sequence of XJ-160 was determined. XJ-160 has diverged significantly from the prototype Sindbis virus, with an 18% difference in nucleotide sequence and an 8ยท6% difference in amino acids; there are 11 deletions and 2 insertions, involving 99 nucleotides in total. XJ-160 is most closely linked to Kyzylagach virus isolated in Azerbaijan. Both belong to the African/European genetic lineage of Sindbis virus, albeit more distantly related to other members., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-81-5-1347