1887

Abstract

A cellular protein that interacts with the NS5A polypeptide of bovine viral diarrhoea virus (BVDV) was identified in a yeast two-hybrid screen. The NS5A interactor was identified as the α subunit of bovine translation elongation factor 1A (eEF1A). Cell-free binding studies were performed with chimeric NS5A fused to glutathione -transferase (GST–NS5A) expressed in bacteria. GST–NS5A bound specifically to both -translated and mammalian cell-expressed eEF1A. Moreover, purified eEF1A bound specifically to GST–NS5A attached to a solid phase. Conservation of this interaction was then analysed using a set of NS5A proteins derived from divergent BVDV strains encompassing known biotypes and genotypes. NS5A from all BVDV strains tested so far interacted with eEF1A. The conserved association of eEF1A with virus molecules involved in genome replication and the postulated role of pestivirus and hepacivirus NS5A in replication indicate that this interaction may play a role in the replication of BVDV.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-82-12-2935
2001-12-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/82/12/0822935a.html?itemId=/content/journal/jgv/10.1099/0022-1317-82-12-2935&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. Journal of Molecular Biology 215:403–410
    [Google Scholar]
  2. Andino R., Rieckhof G. E., Achacoso P. L., Baltimore D. 1993; Poliovirus RNA synthesis utilizes an RNP complex formed around the 5′-end of viral RNA. EMBO Journal 12:3587–3598
    [Google Scholar]
  3. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. 1989 Current Protocols in Molecular Biology, 2nd edn. vol. 1–2 Edited by Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. New York: Greene Publishing Associates and Wiley Interscience;
  4. Bastin M., Hall T. C. 1976; Interaction of elongation factor 1 with aminoacylated brome mosaic virus and tRNAs. Journal of Virology 20:117–122
    [Google Scholar]
  5. Blackwell J. L., Brinton M. A. 1997; Translation elongation factor-1α interacts with the 3′ stem–loop region of West Nile virus genomic RNA. Journal of Virology 71:6433–6444
    [Google Scholar]
  6. Blumenthal T., Landers T. A., Weber K. 1972; Bacteriophage Q replicase contains the protein biosynthesis elongation factors EF Tu and EF Ts. Proceedings of the National Academy of Sciences, USA 69:1313–1317
    [Google Scholar]
  7. Brown D., Gold L. 1996; RNA replication by Qβ replicase: a working model. Proceedings of the National Academy of Sciences, USA 93:11558–11562
    [Google Scholar]
  8. Carvalho J. F., Carvalho M. D., Merrick W. C. 1984; Purification of various forms of elongation factor 1 from rabbit reticulocytes. Archives of Biochemistry and Biophysics 234:591–602
    [Google Scholar]
  9. Cavallius J., Popkie A. P., Merrick W. C. 1997; Site-directed mutants of post-translationally modified sites of yeast eEF1A using a shuttle vector containing a chromogenic switch. Biochimica et Biophysica Acta 1350:345–358
    [Google Scholar]
  10. Collett M. S., Larson R., Gold C., Strick D., Anderson D. K., Purchio A. F. 1988a; Molecular cloning and nucleotide sequence of the pestivirus bovine viral diarrhea virus. Virology 165:191–199
    [Google Scholar]
  11. Collett M. S., Larson R., Belzer S. K., Retzel E. 1988b; Proteins encoded by bovine viral diarrhea virus: the genomic organization of a pestivirus. Virology 165:200–208
    [Google Scholar]
  12. Condeelis J. 1995; Elongation factor 1α, translation and the cytoskeleton. Trends in Biochemical Sciences 20:169–170
    [Google Scholar]
  13. Das T., Mathur M., Gupta A. K., Janssen G. M., Banerjee A. K. 1998; RNA polymerase of vesicular stomatitis virus specifically associates with translation elongation factor-1 αβγ for its activity. Proceedings of the National Academy of Sciences, USA 95:1449–1454
    [Google Scholar]
  14. Deng R., Brock K. V. 1992; Molecular cloning and nucleotide sequence of a pestivirus genome, noncytopathic bovine viral diarrhea virus strain SD-1. Virology 191:867–869
    [Google Scholar]
  15. Deng R., Brock K. V. 1993; 5′ and 3′ untranslated regions of pestivirus genome: primary and secondary structure analyses. Nucleic Acids Research 21:1949–1957
    [Google Scholar]
  16. Donis R. O. 1995; Molecular biology of bovine viral diarrhea virus and its interactions with the host. Veterinary Clinics of North America. Food Animal Practice 11:393–423
    [Google Scholar]
  17. Eigen M., Biebricher C. K., Gebinoga M., Gardiner W. C. 1991; The hypercycle. Coupling of RNA and protein biosynthesis in the infection cycle of an RNA bacteriophage. Biochemistry 30:11005–11018
    [Google Scholar]
  18. Elbers K., Tautz N., Becher P., Stoll D., Rumenapf T., Thiel H. J. 1996; Processing in the pestivirus E2–NS2 region: identification of proteins p7 and E2p7. Journal of Virology 70:4131–4135
    [Google Scholar]
  19. Feng D. F., Doolittle R. F. 1996; Progressive alignment of amino acid sequences and construction of phylogenetic trees from them. Methods in Enzymology 266:368–382
    [Google Scholar]
  20. Gamarnik A. V., Andino R. 1998; Switch from translation to RNA replication in a positive-stranded RNA virus. Genes & Development 12:2293–2304
    [Google Scholar]
  21. Gonen H., Smith C. E., Siegel N. R., Kahana C., Merrick W. C., Chakraburtty K., Schwartz A. L., Ciechanover A. 1994; Protein synthesis elongation factor EF-1α is essential for ubiquitin-dependent degradation of certain N α-acetylated proteins and may be substituted for by the bacterial elongation factor EF-Tu. Proceedings of the National Academy of Sciences, USA 91:7648–7652
    [Google Scholar]
  22. Goodwin J. B., Dreher T. W. 1998; Transfer RNA mimicry in a new group of positive-strand RNA plant viruses, the furoviruses: differential aminoacylation between the RNA components of one genome. Virology 246:170–178
    [Google Scholar]
  23. Gu B., Liu C., Lin-Goerke J., Maley D. R., Gutshall L. L., Feltenberger C. A., Del Vecchio A. M. 2000; The RNA helicase and nucleotide triphosphatase activities of the bovine viral diarrhea virus NS3 protein are essential for viral replication. Journal of Virology 74:1794–1800
    [Google Scholar]
  24. Guan K. L., Dixon J. E. 1991; Eukaryotic proteins expressed in Escherichia coli : an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S -transferase. Analytical Biochemistry 192:262–267
    [Google Scholar]
  25. Gyuris J., Golemis E., Chertkov H., Brent R. 1993; Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2. Cell 75:791–803
    [Google Scholar]
  26. Harris K. S., Xiang W., Alexander L., Lane W. S., Paul A. V., Wimmer E. 1994; Interaction of poliovirus polypeptide 3CDpro with the 5′ and 3′ termini of the poliovirus genome. Identification of viral and cellular cofactors needed for efficient binding. Journal of Biological Chemistry 269:27004–27014
    [Google Scholar]
  27. Janda M., Ahlquist P. 1998; Brome mosaic virus RNA replication protein 1a dramatically increases in vivo stability but not translation of viral genomic RNA3. Proceedings of the National Academy of Sciences, USA 95:2227–2232
    [Google Scholar]
  28. Kruse C., Grunweller A., Willkomm D. K., Pfeiffer T., Hartmann R. K., Muller P. K. 1998; tRNA is entrapped in similar, but distinct, nuclear and cytoplasmic ribonucleoprotein complexes, both of which contain vigilin and elongation factor 1α. Biochemical Journal 329:615–621
    [Google Scholar]
  29. Lai M. M. 1998; Cellular factors in the transcription and replication of viral RNA genomes: a parallel to DNA-dependent RNA transcription. Virology 244:1–12
    [Google Scholar]
  30. Lai V. C., Kao C. C., Ferrari E., Park J., Uss A. S., Wright-Minogue J., Hong Z., Lau J. Y. 1999; Mutational analysis of bovine viral diarrhea virus RNA-dependent RNA polymerase. Journal of Virology 73:10129–10136
    [Google Scholar]
  31. Meyers G., Tautz N., Becher P., Thiel H. J., Kummerer B. M. 1996; Recovery of cytopathogenic and noncytopathogenic bovine viral diarrhea viruses from cDNA constructs. Journal of Virology 70:8606–8613
    [Google Scholar]
  32. Murray J. W., Edmonds B. T., Liu G., Condeelis J. 1996; Bundling of actin filaments by elongation factor 1α inhibits polymerization at filament ends. Journal of Cell Biology 135:1309–1321
    [Google Scholar]
  33. Negrutskii B. S., El’skaya A. V. 1998; Eukaryotic translation elongation factor 1α: structure, expression, functions, and possible role in aminoacyl-tRNA channeling. Progress in Nucleic Acid Research and Molecular Biology 60:47–78
    [Google Scholar]
  34. Nguyen L., Lucas W. J., Ding B., Zaitlin M. 1996; Viral RNA trafficking is inhibited in replicase-mediated resistant transgenic tobacco plants. Proceedings of the National Academy of Sciences, USA 93:12643–12647
    [Google Scholar]
  35. Novak J. E., Kirkegaard K. 1994; Coupling between genome translation and replication in an RNA virus. Genes & Development 8:1726–1737
    [Google Scholar]
  36. Nugent C. I., Johnson K. L., Sarnow P., Kirkegaard K. 1999; Functional coupling between replication and packaging of poliovirus replicon RNA. Journal of Virology 73:427–435
    [Google Scholar]
  37. Reed K. E., Xu J., Rice C. M. 1997; Phosphorylation of the hepatitis C virus NS5A protein in vitro and in vivo : properties of the NS5A-associated kinase. Journal of Virology 71:7187–7197
    [Google Scholar]
  38. Reed K. E., Gorbalenya A. E., Rice C. M. 1998; The NS5A/NS5 proteins of viruses from three genera of the family Flaviviridae are phosphorylated by associated serine/threonine kinases. Journal of Virology 72:6199–6206
    [Google Scholar]
  39. Rumenapf T., Unger G., Strauss J. H., Thiel H. J. 1993; Processing of the envelope glycoproteins of pestiviruses. Journal of Virology 67:3288–3294
    [Google Scholar]
  40. Shiina N., Gotoh Y., Kubomura N., Iwamatsu A., Nishida E. 1994; Microtubule severing by elongation factor 1α. Science 266:282–285
    [Google Scholar]
  41. Slobin L. I. 1980; The role of eucaryotic factor Tu in protein synthesis. The measurement of the elongation factor Tu content of rabbit reticulocytes and other mammalian cells by a sensitive radioimmunoassay. European Journal of Biochemistry 110:555–563
    [Google Scholar]
  42. Sprinzl M. 1994; Elongation factor Tu: a regulatory GTPase with an integrated effector. Trends in Biochemical Sciences 19:245–250
    [Google Scholar]
  43. Tautz N., Elbers K., Stoll D., Meyers G., Thiel H. J. 1997; Serine protease of pestiviruses: determination of cleavage sites. Journal of Virology 71:5415–5422
    [Google Scholar]
  44. van Olphen A. L., Donis R. O. 1997; Identification of bovine viral diarrhea virus nonstructural polypeptide NS4B/P38. Virus Research 51:197–201
    [Google Scholar]
  45. Vassilev V. B., Donis R. O. 2000; Bovine viral diarrhea virus induced apoptosis correlates with increased intracellular viral RNA accumulation. Virus Research 69:95–107
    [Google Scholar]
  46. Wengler G., Bradley D. W., Collett M. S., Heinz F. X., Schlesinger R. W., Strauss J. H. 1995; The Flaviviridae . In Virus Taxonomy. Sixth Report of the International Committee on Taxonomy of Viruses pp 415–427 Edited by Murphy F. A., Fauquet C. M., Bishop D. H. L., Ghabrial S. A., Jarvis A. W., Martelli G. P., Mayo M. A., Summers M. D. Vienna: Springer-Verlag;
    [Google Scholar]
  47. Xu J., Mendez E., Caron P. R., Lin C., Murcko M. A., Collett M. S., Rice C. M. 1997; Bovine viral diarrhea virus NS3 serine proteinase: polyprotein cleavage sites, cofactor requirements, and molecular model of an enzyme essential for pestivirus replication. Journal of Virology 71:5312–5322
    [Google Scholar]
  48. Yang F., Demma M., Warren V., Dharmawardhane S., Condeelis J. 1990; Identification of an actin-binding protein from Dictyostelium as elongation factor 1α. Nature 347:494–496
    [Google Scholar]
  49. Zhong W., Gutshall L. L., Del Vecchio A. M. 1998; Identification and characterization of an RNA-dependent RNA polymerase activity within the nonstructural protein 5B region of bovine viral diarrhea virus. Journal of Virology 72:9365–9369
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-82-12-2935
Loading
/content/journal/jgv/10.1099/0022-1317-82-12-2935
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error