1887

Abstract

DNA vaccines have several advantages over conventional vaccines. One of the most important characteristics is the presentation of antigen via both MHC class I and class II receptors. Although this generally results in strong T-cell responses, antibody production and protection achieved by DNA immunization are unfortunately not always adequate. In contrast, modified live virus (MLV) vaccines usually induce adequate antibody and moderate cellular responses, whereas killed vaccines tend to elicit weak immune responses in general. A DNA prime–MLV boost regimen should result in enhanced cellular immunity and possibly improved antibody production. To test this hypothesis, plasmids encoding bovine herpesvirus-1 (BHV-1) glycoproteins B and D were delivered by gene gun to the genital mucosa of cattle prior to immunization with modified live BHV-1 vaccine. The immune responses induced were compared to those of an MLV-vaccinated group and a negative control group. Although significantly enhanced T-cell responses were induced by priming with the DNA vaccine, there was no increase in antibody titres. Similar levels of protection were induced by the MLV vaccine alone and the DNA prime and MLV boost regimen, which suggests that there is no correlation between the induction of T-cell responses and protection from BHV-1 challenge.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-82-12-3035
2001-12-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/82/12/0823035a.html?itemId=/content/journal/jgv/10.1099/0022-1317-82-12-3035&mimeType=html&fmt=ahah

References

  1. Babiuk L. A., van Drunen Littel-van den Hurk S., Tikoo S. K. 1996; Immunology of bovine herpesvirus 1 infection. Veterinary Microbiology 53:31–42
    [Google Scholar]
  2. Braun R. P., Babiuk L. A., van Drunen Littel-van den Hurk S. 1997; Enhanced immune responses to an intradermally delivered DNA vaccine expressing a secreted form of BHV-1 gD. Vaccine Research 6:151–164
    [Google Scholar]
  3. Braun R., Babiuk L. A., van Drunen Littel-van den Hurk S. 1998; Compatibility of plasmids expressing different antigens in a single DNA vaccine formulation. Journal of General Virology 79:2965–2970
    [Google Scholar]
  4. Braun R. P., Babiuk L. A., Loehr B. I., van Drunen Littel-van den Hurk S. 1999; Particle-mediated DNA immunization of cattle confers long-lasting immunity against bovine herpesvirus-1 . Virology 26546-56
    [Google Scholar]
  5. Chen C. H., Ji H., Suh K. W., Choti M. A., Pardoll D. M., Wu T. C. 1999; Gene gun-mediated DNA vaccination induces antitumor immunity against human papillomavirus type 16 E7-expressing murine tumor metastases in the liver and lungs. Gene Therapy 6:1972–1981
    [Google Scholar]
  6. Degano P., Schneider J., Hannan C. M., Gilbert S. C., Hill A. V. 1999; Gene gun intradermal DNA immunization followed by boosting with modified vaccinia virus Ankara: enhanced CD8+ T cell immunogenicity and protective efficacy in the influenza and malaria models. Vaccine 12:623–632
    [Google Scholar]
  7. de Zoeten E., Carr-Brendel V., Markovic D., Taylor-Papadimitriou J., Cohen E. P. 1999; Treatment of breast cancer with fibroblasts transfected with DNA from breast cancer cells. Journal of Immunology 162:6934–6941
    [Google Scholar]
  8. Donnelly J. J., Ulmer J. B., Liu M. A. 1997; DNA vaccines. Annual Review of Immunology 15:617–648
    [Google Scholar]
  9. Fensterle J., Grode L., Hess J., Kaufmann S. H. 1999; Effective DNA vaccination against listeriosis by prime/boost inoculation with the gene gun. Journal of Immunology 163:4510–4518
    [Google Scholar]
  10. Haddad D., Liljeqvist S., Stahl S., Hansson M., Perlmann P., Ahlborg N., Berzins K. 1999; Characterization of antibody responses to a Plasmodium falciparum blood-stage antigen induced by a DNA prime/protein boost immunization protocol. Scandinavian Journal of Immunology 49:506–514
    [Google Scholar]
  11. Hanke T., Samuel R. V., Blanchard T. J., Neumann V. C., Allen T. M., Boyson J. E., Sharpe S. A., Cook N., Smith G. L., Watkins D. L., Cranage M. P., McMichael A. J. 1999; Effective induction of simian immunodeficiency virus-specific cytotoxic T lymphocytes in macaques by using a multiepitope gene and DNA prime–modified vaccinia virus Ankara boost vaccination regimen. Journal of Virology 73:7524–7532
    [Google Scholar]
  12. Hassett D. E., Zhang J., Slifka M., Whitton J. L. 2000; Immune responses following neonatal DNA vaccination are long-lived, abundant, and qualitatively similar to those induced by conventional immunization. Journal of Virology 74:2620–2627
    [Google Scholar]
  13. Ji H., Wang T. L., Chen C. H., Pai S. I., Hung C. F., Lin K. Y., Kurman R. J., Pardoll D. M., Wu T. C. 1999; Targeting human papillomavirus type 16 E7 to the endosomal/lysosomal compartment enhances the antitumor immunity of DNA vaccines against murine human papillomavirus type 16 E7-expressing tumors. Human Gene Therapy 10:2727–2740
    [Google Scholar]
  14. Johnson P. A., Conway M. A., Daly J., Nicolson C., Robertson J., Mills K. H. G. 2000; Plasmid DNA encoding influenza virus haemagglutinin induces Th1 cells and protection against respiratory infection despite its limited ability to generate antibody responses. Journal of General Virology 81:1737–1745
    [Google Scholar]
  15. Kowalski J. K., Gilbert S. A., van Drunen Littel-van den Hurk S., van den Hurk J. V., Babiuk L. A., Zamb T. J. 1993; Heat-shock promoter-driven synthesis of secreted bovine herpesvirus glycoproteins in transfected cells. Vaccine 11:1100–1107
    [Google Scholar]
  16. Lasaro M. O., Alves A. M., Guillobel H. C., Almeida D. F., Ferreira L. C. 1999; New vaccine strategies against enterotoxigenic Escherichia coli. II. Enhanced systemic and secreted antibody responses against the CFA/I fimbriae by priming with DNA and boosting with a live recombinant Salmonella vaccine. Brazilian Journal of Medical and Biological Research 32:241–246
    [Google Scholar]
  17. Laylor R., Porakishvili N., de Souza J. B., Playfair J. H., Delves P. J., Lund T. 1999; DNA vaccination favours memory rather than effector B cell responses. Clinical and Experimental Immunology 117:106–112
    [Google Scholar]
  18. Li Y., van Drunen Littel-van den Hurk S., Liang X., Babiuk L. A. 1996; Production and characterization of bovine herpesvirus 1 glycoprotein B ectodomain derivatives in an hsp70A gene promoter-based expression system. Archives of Virology 141:2019–2029
    [Google Scholar]
  19. Loehr B. I., Babiuk L. A., Willson P., van Drunen Littel-van den Hurk S. 2000; Gene gun mediated DNA immunization primes the development of mucosal immunity against bovine herpesvirus-1 in cattle. Journal of Virology 74:6077–6086
    [Google Scholar]
  20. Ohwada A., Sekiya M., Hanaki H., Arai K. K., Nagaoka I., Hori S., Tominaga S., Hiramatsu K., Fukuchi Y. 1999; DNA vaccination by mecA sequence evokes an antibacterial immune response against methicillin-resistant Staphylococcus aureus. Journal of Antimicrobial Chemotherapy 44:767–774
    [Google Scholar]
  21. Park J. H., Kim C. J., Lee J. H., Shin S. H., Chung G. H., Jang Y. S. 1999; Effective immunotherapy of cancer by DNA vaccination. Molecules and Cells 9:384–391
    [Google Scholar]
  22. Robinson H. L., Montefiori D. C., Johnson R. P., Manson K. H., Kalish M. L., Lifson J. D., Rizvi T. A., Lu S., Hu S. L., Mazzara G. P., Panicali D. L., Herndon J. G., Glickman R., Candido M. A., Lydy S. L., Wyand M. S., McClure H. M. 1999; Neutralizing antibody-independent containment of immunodeficiency virus challenges by DNA priming and recombinant pox virus booster immunizations. Nature Medicine 5:526–534
    [Google Scholar]
  23. Ruitenberg K. M., Walker C., Love D. N., Wellington J. E., Whalley J. M. 2000; A prime–boost immunization strategy with DNA and recombinant baculovirus-expressed protein enhances protective immunogenicity of glycoprotein D of equine herpesvirus 1 in naive and infection-primed mice. Vaccine 31:1367–1373
    [Google Scholar]
  24. Schneider J., Gilbert S. C., Hannan C. M., Degano O., Prieur E., Sheu E. G., Plebanski M., Hill A. V. 1999; Induction of CD8+ T cells using heterologous prime–boost immunisation strategies. Immunological Reviews 170:29–38
    [Google Scholar]
  25. Sin J. I., Bagarazzi M., Pachuk C., Weiner D. B. 1999; DNA priming-protein boosting enhances both antigen-specific antibody and Th1-type cellular immune responses in a murine herpes simplex virus-2 gD vaccine model. DNA and Cell Biology 18:771–779
    [Google Scholar]
  26. Tan J., Yang N. S., Turner J. G., Niu G. L., Maassab H. F., Sun J., Herlocher M. L., Chang A. E., Yu H. 1999; Interleukin-12 cDNA skin transfection potentiates human papillomavirus E6 DNA vaccine-induced antitumor immune response. Cancer Gene Therapy 6:331–339
    [Google Scholar]
  27. Tikoo S. K., Fitzpatrick D., Babiuk L. A., Zamb T. J. 1990; Molecular cloning, sequencing and expression of functional bovine herpesvirus-1 glycoprotein gIV in transfected bovine cells. Journal of Virology 64:5132–5142
    [Google Scholar]
  28. Turin L., Russo S., Poli G. 1999; BHV-1: new molecular approaches to control a common and widespread infection. Molecular Medicine 5:261–284
    [Google Scholar]
  29. Van Donkersgoed J., Babiuk L. A. 1991; Diagnosing and managing the respiratory form of infectious bovine rhinotracheitis. Veterinary Medicine 1:86–94
    [Google Scholar]
  30. Van Donkersgoed J., Klassen P. 1995; Serological study of a modified-live virus IBR vaccine given to feedlot calves after arrival. Canadian Veterinary Journal 36:394
    [Google Scholar]
  31. van Drunen Littel-van den Hurk S., Gifford G. A., Babiuk L. A. 1990; The epitope specificity of the protective immune response induced by individual bovine herpesvirus-1 (BHV-1) glycoproteins. Vaccine 8:358–368
    [Google Scholar]
  32. van Drunen Littel-van den Hurk S., Braun R. P., Lewis P. J., Karvonen B. C., Baca-Estrada M. E., Snider M., McCartney D., Watts T., Babiuk L. A. 1998; Intradermal immunization with a bovine herpesvirus-1 DNA vaccine induces protective immunity in cattle. Journal of General Virology 79:831–839
    [Google Scholar]
  33. Wei W. Z., Shi W. P., Galy A., Lichlyter D., Hernandez S., Groner B., Heilbrun L., Jones R. F. 1999; Protection against mammary tumor growth by vaccination with full-length, modified human ErbB-2 DNA. International Journal of Cancer 81:748–754
    [Google Scholar]
  34. Yates W. D. G. 1982; A review of infectious bovine rhinotracheitis, shipping fever pneumonia and viral-bacterial synergism in respiratory disease of cattle. Canadian Journal of Comparative Medicine 46:225–263
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-82-12-3035
Loading
/content/journal/jgv/10.1099/0022-1317-82-12-3035
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error