%0 Journal Article %A Khattar, Sunil K. %A Yunus, Abdul S. %A Samal, Siba K. %T Mapping the domains on the phosphoprotein of bovine respiratory syncytial virus required for N–P and P–L interactions using a minigenome system %D 2001 %J Journal of General Virology, %V 82 %N 4 %P 775-779 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-82-4-775 %I Microbiology Society, %X The interaction of bovine respiratory syncytial virus (BRSV) phosphoprotein (P) with nucleocapsid (N) and large polymerase (L) proteins was investigated using an intracellular BRSV–CAT minigenome replication system. Coimmunoprecipitation assays using P-specific antiserum revealed that the P protein can form complexes with N and L proteins. Deletion mutant analysis of the P protein was performed to identify the regions of P protein that interact with N and L proteins. The results indicate that two independent N-binding sites exist on the P protein: an internal region of 161–180 amino acids and a C-terminal region of 221–241 amino acids. The L-binding site was mapped to a region of P protein encompassing amino acids 121–160. The data suggest that N and L protein binding domains on the P protein do not overlap. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-82-4-775