
f Poliovirus transcytosis through M-like cells
- Authors: Laurent Ouzilou1 , Elise Caliot1 , Isabelle Pelletier1 , Marie-Christine Prévost1 , Eric Pringault1 , Florence Colbère-Garapin1
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1 Génétique Virale-NRSN, Département Neuroscience1, Interactions Lymphoépithéliales2 and Plate-forme de microscopie électronique3, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris cedex 15, France
- Author for correspondence: Florence Colbère-Garapin. Fax +33 1 40 61 33 67. e-mail [email protected]
- First Published Online: 01 September 2002, Journal of General Virology 83: 2177-2182, doi: 10.1099/0022-1317-83-9-2177
- Subject: Animal: RNA Viruses
- Received:
- Accepted:
- Cover date:




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During the digestive-tract phase of infection, poliovirus (PV) is found in the oropharynx and the intestine. It has been proposed that PV enters the organism by crossing M cells, which are scattered in the epithelial sheet covering lymphoid follicles of Peyer’s patches. However, PV translocation through M cells has never been demonstrated. A model of M-like cells has been previously developed using monolayers of polarized Caco-2 enterocytes cocultured with lymphocytes isolated from Peyer’s patches. In this model, lymphoepithelial interactions trigger the appearance of epithelial cells having morphological and functional characteristics of M cells. We have demonstrated efficient, temperature-dependent PV transcytosis in Caco-2 cell monolayers containing M-like cells. This experimental evidence is consistent with M cells serving as gateways allowing PV access to the basal face of enterocytes, the underlying immune follicle cells, and PV transport toward mesenteric lymph nodes.
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