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f Poliovirus transcytosis through M-like cells

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  • During the digestive-tract phase of infection, poliovirus (PV) is found in the oropharynx and the intestine. It has been proposed that PV enters the organism by crossing M cells, which are scattered in the epithelial sheet covering lymphoid follicles of Peyer’s patches. However, PV translocation through M cells has never been demonstrated. A model of M-like cells has been previously developed using monolayers of polarized Caco-2 enterocytes cocultured with lymphocytes isolated from Peyer’s patches. In this model, lymphoepithelial interactions trigger the appearance of epithelial cells having morphological and functional characteristics of M cells. We have demonstrated efficient, temperature-dependent PV transcytosis in Caco-2 cell monolayers containing M-like cells. This experimental evidence is consistent with M cells serving as gateways allowing PV access to the basal face of enterocytes, the underlying immune follicle cells, and PV transport toward mesenteric lymph nodes.

  • Amerongen, H. M., Wilson, G. A., Fields, B. N. & Neutra, M. R. ( 1994; ). Proteolytic processing of reovirus is required for adherence to intestinal M cells. Journal of Virology 68, 8428-8432.
     [Google Scholar]
  • Arita, M., Ohka, S., Sasaki, Y. & Nomoto, A. ( 1999; ). Multiple pathways for establishment of poliovirus infection. Virus Research 62, 97-105.[CrossRef]
    [Google Scholar]
  • Bass, D. M., Trier, J. S., Dambrauskas, R. & Wolf, J. L. ( 1988; ). Reovirus type I infection of small intestinal epithelium in suckling mice and its effect on M cells. Laboratory Investigation 58, 226-235.
     [Google Scholar]
  • Blondel, B., Duncan, G., Couderc, T., Delpeyroux, F., Pavio, N. & Colbère-Garapin, F. ( 1998; ). Molecular aspects of poliovirus biology with a special focus on the interactions with nerve cells. Journal of Neurovirology 4, 1-26.[CrossRef]
    [Google Scholar]
  • Bodian, D. ( 1955; ). Emerging concept of poliomyelitis infection. Science 122, 105-108.[CrossRef]
    [Google Scholar]
  • Costa de Beauregard, M. A., Pringault, E., Robine, S. & Louvard, D. ( 1995; ). Suppression of villin expression by antisense RNA impairs brush border assembly in polarized epithelial intestinal cells. EMBO Journal 14, 409-421.
     [Google Scholar]
  • Dickinson, B. L., Badizadegan, K., Wu, Z., Ahouse, J. C., Zhu, X., Simister, N. E., Blumberg, R. S. & Lencer, W. I. ( 1999; ). Bidirectional FcRn-dependent IgG transport in a polarized human intestinal epithelial cell line. Journal of Clinical Investigation 104, 903-911.[CrossRef]
    [Google Scholar]
  • El Bahi, S., Caliot, E., Bens, M., Bogdanova, A., Kerneis, S., Kahn, A., Vandewalle, A. & Pringault, E. ( 2002; ). Lympho-epithelial interactions trigger specific regulation of gene expression in the M-cell containing follicle-associated epithelium of Peyer’s patches. Journal of Immunology 168, 3713-3720.[CrossRef]
    [Google Scholar]
  • Emmanuel, A. V., Mason, H. J. & Kamm, M. A. ( 2001; ). Relationship between psychological state and level of activity of extrinsic gut innervation in patients with a functional gut disorder. Gut 49, 209-213.[CrossRef]
    [Google Scholar]
  • Gebert, A. ( 1996; ). M-cells in the rabbit tonsil exhibit distinctive glycoconjugates in their apical membranes. Journal of Histochemistry and Cytochemistry 44, 1033-1042.[CrossRef]
    [Google Scholar]
  • Gullberg, E. M., Leonard, M., Karlsson, J., Hopkins, A. M., Brayden, D., Baird, A. W. & Artursson, P. ( 2000; ). Expression of specific markers and particle transport in a new human intestinal M-cell model. Biochemical and Biophysical Research Communications 279, 803-813.[CrossRef]
    [Google Scholar]
  • Heppner, F. L., Christ, A. D., Klein, M. A., Prinz, M., Fried, M., Kraehenbuhl, J. P. & Aguzzi, A. ( 2001; ). Transepithelial prion transport by M cells. Nature Medicine 7, 976-977.[CrossRef]
    [Google Scholar]
  • Kerneis, S., Bogdanova, A., Kraehenbuhl, J. P. & Pringault, E. ( 1997; ). Conversion by Peyer’s patch lymphocytes of human enterocytes into M cells that transport bacteria. Science 277, 949-952.[CrossRef]
    [Google Scholar]
  • Kerneis, S., Caliot, E., Stubbe, H., Bogdanova, A., Kraehenbuhl, J. & Pringault, E. ( 2000; ). Molecular studies of the intestinal mucosal barrier physiopathology using cocultures of epithelial and immune cells: a technical update. Microbes and Infection 2, 1119-1124.[CrossRef]
    [Google Scholar]
  • Kraehenbuhl, J. P. & Neutra, M. R. ( 1992; ). Molecular and cellular basis of immune protection of mucosal surfaces. Physiological Reviews 72, 853-879.
     [Google Scholar]
  • Lopez, M., Jordier, F., Bardin, F., Coulombel, L., Chabannon, C. & Dubreuil, P. ( 1999; ). Identification of a new class of Ig superfamily antigens expressed in hemopoiesis. In Leucocyte Typing VI. White Cell Differentiation Antigens , pp. 1081. Edited by T. K. Kishimoto, H. Kikutani, A. E. G. von dem Borne, S. M. Goyert, D. Y. Mason, M. Miyasaka, M. Moretta, K. Okumura, S. Shaw, T. A. Springer, K. Sugamura & H. Zola. New York: Garland Publishing.
  • Madara, J. L. & Trier, J. S. ( 1994; ). The functional morphology of the mucosa of the small intestine. In Physiology of the Gastrointestinal Tract , pp. 1577. Edited by L. R. Johnson. New York: Raven Press.
  • Madara, J. L., Bye, W. A. & Trier, J. S. ( 1984; ). Structural features of and cholesterol distribution in M-cell membranes in guinea pig, rat and mouse Peyer’s patches. Gastroenterology 87, 1091-1103.
     [Google Scholar]
  • Minor, P. D. ( 1997; ). Poliovirus. In Viral Pathogenesis , pp. 555-574. Edited by N. Nathanson. Philadelphia: Lippincott–Raven.
  • Mostov, K. E., Verges, M. & Altschuler, Y. ( 2000; ). Membrane traffic in polarized epithelial cells. Current Opinion in Cell Biology 12, 483-490.[CrossRef]
    [Google Scholar]
  • Neutra, M. R., Frey, A. & Kraehenbuhl, J. P. ( 1996a; ). Epithelial M cells: gateways for mucosal infection and immunization. Cell 86, 345-348.[CrossRef]
    [Google Scholar]
  • Neutra, M. R., Pringault, E. & Kraehenbuhl, J. P. ( 1996b; ). Antigen sampling across epithelial barriers and induction of mucosal immune responses. Annual Review of Immunology 14, 275-300.[CrossRef]
    [Google Scholar]
  • Niedergang, F. & Kraehenbuhl, J. P. ( 2000; ). Much ado about M cells. Trends in Cell Biology 10, 137-141.[CrossRef]
    [Google Scholar]
  • Owen, R. L. ( 1977; ). Sequential uptake of horseradish peroxidase by lymphoid follicle epithelium of Peyer’s patches in the normal unobstructed mouse intestine: an ultrastructural study. Gastroenterology 72, 440-451.
     [Google Scholar]
  • Owen, R. L. & Jones, A. L. ( 1974; ). Epithelial cell specialization within human Peyer’s patches: an ultrastructural study of intestinal lymphoid follicles. Gastroenterology 66, 189-203.
     [Google Scholar]
  • Pavio, N., Buc-Caron, M. H. & Colbère-Garapin, F. ( 1996; ). Persistent poliovirus infection of human fetal brain cells. Journal of Virology 70, 6395-6401.
     [Google Scholar]
  • Pinto, M., Robine-Leon, S., Appay, M., Kedinger, M., Triadou, N., Dussaulx, E., Lacroix, B., Simon-Assmann, P., Haffen, K., Fogh, J. & Zweibaum, A. ( 1983; ). Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture. Biology of the Cell 47, 323-330.
     [Google Scholar]
  • Racaniello, V. & Ren, R. ( 1996; ). Poliovirus biology and pathogenesis. Current Topics in Microbiology and Immunology 206, 305-325.
     [Google Scholar]
  • Sicinski, P., Rowinski, J., Warchol, J. B., Jarzabek, Z., Gut, W., Szczygiel, B., Bielecki, K. & Koch, G. ( 1990; ). Poliovirus type 1 enters the human host through intestinal M cells. Gastroenterology 98, 56-58.
     [Google Scholar]
  • Siebers, A. & Finlay, B. B. ( 1996; ).  M cells and the pathogenesis of mucosal and systemic infections. Trends in Microbiology 4, 22-29.[CrossRef]
    [Google Scholar]
  • Tucker, S. P., Thornton, C. L., Wimmer, E. & Compans, R. W. ( 1993a; ). Bidirectional entry of poliovirus into polarized epithelial cells. Journal of Virology 67, 29-38.
     [Google Scholar]
  • Tucker, S. P., Thornton, C. L., Wimmer, E. & Compans, R. W. ( 1993b; ). Vectorial release of poliovirus from polarized human intestinal epithelial cells. Journal of Virology 67, 4274-4282.
     [Google Scholar]
  • Wimmer, E., Hellen, C. U. & Cao, X. M. ( 1993; ). Genetics of poliovirus. Annual Review of Genetics 27, 353-436.[CrossRef]
    [Google Scholar]
  • Wolf, J. L., Rubin, D. H., Finberg, R., Kauffman, R. S., Sharpe, A. H., Trier, J. S. & Fields, B. N. ( 1981; ). Intestinal M cells: a pathway for entry of reovirus into the host. Science 212, 471-472.[CrossRef]
    [Google Scholar]
  • Wolf, J. L., Dambrauskas, R., Sharpe, A. H. & Trier, J. S. ( 1987; ). Adherence to and penetration of the intestinal epithelium by reovirus type 1 in neonatal mice. Gastroenterology 92, 82-91.
     [Google Scholar]
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2002-09-01
2019-02-22
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