@article{mbs:/content/journal/jgv/10.1099/jgv.0.000350, author = "Manoharan, Vinoth K. and Khattar, Sunil K. and Paldurai, Anandan and Kim, Shin-Hee and Samal, Siba K.", title = "A Y527A mutation in the fusion protein of Newcastle disease virus strain LaSota leads to a hyperfusogenic virus with increased replication and immunogenicity", journal= "Journal of General Virology", year = "2016", volume = "97", number = "2", pages = "287-292", doi = "https://doi.org/10.1099/jgv.0.000350", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000350", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Newcastle disease is a highly contagious and economically important disease of poultry. Low-virulence Newcastle disease virus (NDV) strains such as B1 and LaSota have been used as live vaccines, with a proven track record of safety and efficacy. However, these vaccines do not completely prevent infection or virus shedding. Therefore, there is a need to enhance the immunogenicity of these vaccine strains. In this study, the effect of mutations in the conserved tyrosine residues of the F protein of vaccine strain LaSota was investigated. Our results showed that substitution of tyrosine at position 527 by alanine resulted in a hyperfusogenic virus with increased replication and immunogenicity. Challenge study with highly virulent NDV strain Texas GB showed that immunization of chickens with Y527A mutant virus provided 100 % protection and no shedding of the challenge virus. This study suggests that the strain LaSota harbouring the Y527A mutation may represent a more efficacious vaccine.", }