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Volume 97, Issue 3

Human parainfluenza virus type 2 V protein inhibits and antagonizes tetherin Free

Abstract

Tetherin (BST-2/CD317/HM1.24) is an antiviral membrane protein that prevents the release of enveloped viruses from the cell surface. We found that the growth of human parainfluenza virus type 2 (hPIV-2), but not that of V protein-deficient recombinant hPIV-2, was inhibited by tetherin. V protein immunoprecipitates with tetherin, and this interaction requires its C-terminal Trp residues. The glycosyl phosphatidylinositol attachment signal of tetherin, but not its cytoplasmic tail, was necessary for its binding with V. The distribution of the V protein clearly changed when co-expressed with tetherin in plasmid-transfected cells. hPIV-2 infection of HeLa cells reduced cell surface tetherin without affecting total cellular tetherin. This reduction also occurred in HeLa cells constitutively expressing V, whereas mutated V protein did not affect the cell surface tetherin. Our results suggest that hPIV-2 V protein antagonizes tetherin by binding it and reducing its presence at the cell surface.

  • Received:
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© 2015 The Authors
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2016-03-01
2024-04-19
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Human parainfluenza virus type 2 V protein inhibits and antagonizes tetherin
J. Gen. Virol. 97, 561 (2016); https://doi.org/10.1099/jgv.0.000373
/content/journal/jgv/10.1099/jgv.0.000373
/content/journal/jgv/10.1099/jgv.0.000373
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