1887

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Volume 97, Issue 7

Poly(ADP-ribose) polymerase-1 silences retroviruses independently of viral DNA integration or heterochromatin formation Free

Abstract

PARP-1 silences retrotransposons in , through heterochromatin maintenance, and integrated retroviruses in chicken. Here, we determined the role of viral DNA integration and cellular heterochromatin in PARP-1-mediated retroviral silencing using HIV-1-derived lentiviral vectors and Rous-associated virus type 1 (RAV-1) as models. Analysis of the infection of PARP-1 knockout and control cells with HIV-1 harbouring WT integrase, in the presence or absence of an integrase inhibitor, or catalytic-dead mutant integrase indicated that silencing does not require viral DNA integration. The mechanism involves the catalytic activity of histone deacetylases but not that of PARP-1. In contrast to , lack of PARP-1 in avian cells did not affect chromatin compaction globally or at the RAV-1 provirus, or the cellular levels of histone H3 N-terminal acetylated or Lys27 trimethylated, as indicated by micrococcal nuclease accessibility and immunoblot assays. Therefore, PARP-1 represses retroviruses prior to viral DNA integration by mechanisms involving histone deacetylases but not heterochromatin formation.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): heterochromatin , histone deacetylases , histone H3 post-translational modifications , PARP-1 , PARP-1 catalytic activity and retroviral silencing
© 2016 The Authors
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2016-07-01
2024-04-20
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Poly(ADP-ribose) polymerase-1 silences retroviruses independently of viral DNA integration or heterochromatin formation
J. Gen. Virol. 97, 1686 (2016); https://doi.org/10.1099/jgv.0.000466
/content/journal/jgv/10.1099/jgv.0.000466
/content/journal/jgv/10.1099/jgv.0.000466
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