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Volume 97, Issue 12

Recombinant rabies virus expressing IL-21 enhances immunogenicity through activation of T follicular helper cells and germinal centre B cells Free

Abstract

Previous studies have demonstrated that the lack of interleukin-21 (IL-21) signalling could affect specific antibody induction after rabies vaccination. Here, to further investigate the over-expression of IL-21 on the immunogenicity of rabies virus (RABV), a recombinant RABV expressing murine IL-21, designated LBNSE-IL21, was constructed and evaluated in a mouse model. It was found that in mice immunized with LBNSE-IL21, there was a substantial increase in the number of T follicular helper cells and germinal centre B cells but no enhancement of dendritic cell activation. Furthermore, significantly higher rabies virus-neutralizing antibody (VNA) titres were produced in mice immunized with LBNSE-IL21 than in mice immunized with the parent virus LBNSE in the first six weeks, resulting in higher protection. Together, these results suggest that LBNSE-IL21 can induce a rapid and robust VNA titre, and it has the potential to be developed as a promising rabies vaccine.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): germinal center B cells , IL-21 , immunogenicity , rabies virus and T follicular helper cells
© 2016 The Authors
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/content/journal/jgv/10.1099/jgv.0.000646
2016-12-16
2024-04-20
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Recombinant rabies virus expressing IL-21 enhances immunogenicity through activation of T follicular helper cells and germinal centre B cells
J. Gen. Virol. 97, 3154 (2016); https://doi.org/10.1099/jgv.0.000646
/content/journal/jgv/10.1099/jgv.0.000646
/content/journal/jgv/10.1099/jgv.0.000646
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