Recombinant rabies virus expressing IL-21 enhances immunogenicity through activation of T follicular helper cells and germinal centre B cells
Zhang, Yajing and Zhou, Ming and Wang, Zhao and Yang, Jie and Li, Mingming and Wang, Kunlun and Cui, Min and Chen, Huanchun and Fu, Zhen F. and Zhao, Ling,, 97, 3154-3160 (2016),
doi = https://doi.org/10.1099/jgv.0.000646,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= Previous studies have demonstrated that the lack of interleukin-21 (IL-21) signalling could affect specific antibody induction after rabies vaccination. Here, to further investigate the over-expression of IL-21 on the immunogenicity of rabies virus (RABV), a recombinant RABV expressing murine IL-21, designated LBNSE-IL21, was constructed and evaluated in a mouse model. It was found that in mice immunized with LBNSE-IL21, there was a substantial increase in the number of T follicular helper cells and germinal centre B cells but no enhancement of dendritic cell activation. Furthermore, significantly higher rabies virus-neutralizing antibody (VNA) titres were produced in mice immunized with LBNSE-IL21 than in mice immunized with the parent virus LBNSE in the first six weeks, resulting in higher protection. Together, these results suggest that LBNSE-IL21 can induce a rapid and robust VNA titre, and it has the potential to be developed as a promising rabies vaccine.,
language=,
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