RT Journal Article SR Electronic(1) A1 Henderson, Davin M. A1 Tennant, Joanne M. A1 Haley, Nicholas J. A1 Denkers, Nathaniel D. A1 Mathiason, Candace K. A1 Hoover, Edward A.YR 2017 T1 Detection of chronic wasting disease prion seeding activity in deer and elk feces by real-time quaking-induced conversion JF Journal of General Virology, VO 98 IS 7 SP 1953 OP 1962 DO https://doi.org/10.1099/jgv.0.000844 PB Microbiology Society, SN 1465-2099, AB Chronic wasting disease (CWD) is an emergent prion disease affecting cervid species in North America, Canada, South Korea, and recently, Norway. Detection of CWD has been advanced by techniques that rely on amplification of low levels of prion amyloid to a detectable level. However, the increased sensitivity of amplification assays is often compromised by inhibitors and/or activators in complex biologic samples including body fluids, excreta, or the environment. Here, we adapt real-time quaking-induced conversion conditions to specifically detect CWD prions in fecal samples from both experimentally infected deer and naturally infected elk and estimate environmental contamination. The results have application to detection, surveillance and management of CWD, and potentially to other protein-misfolding diseases., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000844