Isolation and characterization of a new iflavirus from Armigeres spp. mosquitoes in the Philippines Kobayashi, Daisuke and Isawa, Haruhiko and Fujita, Ryosuke and Murota, Katsunori and Itokawa, Kentaro and Higa, Yukiko and Katayama, Yukie and Sasaki, Toshinori and Mizutani, Tetsuya and Iwanaga, Shiroh and Ohta, Nobuo and Garcia-Bertuso, Arlene and Sawabe, Kyoko,, 98, 2876-2881 (2017), doi = https://doi.org/10.1099/jgv.0.000929, publicationName = Microbiology Society, issn = 0022-1317, abstract= During an entomological surveillance for arthropod-borne viruses in the Philippines, we isolated a previously unrecognized virus from female Armigeres spp. mosquitoes. Whole-genome sequencing, genetic characterization and phylogenetic analysis revealed that the isolated virus, designated Armigeres iflavirus (ArIFV), is a novel member of the iflaviruses (genus Iflavirus, family Iflaviridae) and phylogenetically related to Moku virus, Hubei odonate virus 4, slow bee paralysis virus and Graminella nigrifrons virus 1. To our knowledge, this is the first successful isolation of iflavirus from a dipteran insect. Spherical ArIFV particles of approximately 30 nm in diameter contained at least three major structural proteins. ArIFV multiplied to high titres (~109 p.f.u. ml−1) and formed clear plaques in a mosquito cell line, C6/36. Our findings provide new insights into the infection mechanism, genetic diversity and evolution of the Iflaviridae family., language=, type=