RT Journal Article SR Electronic(1) A1 Kobayashi, Daisuke A1 Isawa, Haruhiko A1 Fujita, Ryosuke A1 Murota, Katsunori A1 Itokawa, Kentaro A1 Higa, Yukiko A1 Katayama, Yukie A1 Sasaki, Toshinori A1 Mizutani, Tetsuya A1 Iwanaga, Shiroh A1 Ohta, Nobuo A1 Garcia-Bertuso, Arlene A1 Sawabe, KyokoYR 2017 T1 Isolation and characterization of a new iflavirus from Armigeres spp. mosquitoes in the Philippines JF Journal of General Virology, VO 98 IS 11 SP 2876 OP 2881 DO https://doi.org/10.1099/jgv.0.000929 PB Microbiology Society, SN 1465-2099, AB During an entomological surveillance for arthropod-borne viruses in the Philippines, we isolated a previously unrecognized virus from female Armigeres spp. mosquitoes. Whole-genome sequencing, genetic characterization and phylogenetic analysis revealed that the isolated virus, designated Armigeres iflavirus (ArIFV), is a novel member of the iflaviruses (genus Iflavirus, family Iflaviridae) and phylogenetically related to Moku virus, Hubei odonate virus 4, slow bee paralysis virus and Graminella nigrifrons virus 1. To our knowledge, this is the first successful isolation of iflavirus from a dipteran insect. Spherical ArIFV particles of approximately 30 nm in diameter contained at least three major structural proteins. ArIFV multiplied to high titres (~109 p.f.u. ml−1) and formed clear plaques in a mosquito cell line, C6/36. Our findings provide new insights into the infection mechanism, genetic diversity and evolution of the Iflaviridae family., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000929