1887

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Volume 99, Issue 5

The interaction of major coat protein with ankyrin repeat protein NbANKr redirects it to chloroplasts and modulates virus infection Free

Abstract

The (LoLV) major coat protein sequence contains a typical chloroplast transit peptide (cTP) domain. In infected leaf tissue, LoLV coat proteins can be detected at the chloroplast. In transient expression, several N-terminal deletions of the CP sequence, increasing in length, result in disruption of the domain functionality, markedly affecting intracellular localization. A yeast two-hybrid-based study using LoLV CP as bait identified several potentially interacting host proteins, most of them with chloroplast-linked pathways. One of them, an ankyrin repeat protein, was studied in detail. The homologue (NbANKr) targets chloroplasts, is able to co-localize with LoLV CP at chloroplast membranes in transient expression and shows a robust interaction with LoLV CP by BiFC, which has been confirmed by yeast two-hybrid data. Silencing NbANKr genes in plants, prior to challenging with LoLV by mechanical inoculation, affects LoLV infection, significantly reducing the level of viral RNA in young leaves, compared to levels in control plants, and suggesting an inhibition of virus movement. Silencing of NbANKr has no obvious effect on plant phenotype, but is able to interfere with LoLV infection, opening the way for a new strategy for virus infection control.

  • Received:
  • Accepted:
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Keyword(s): Ankyrin repeat protein , chloroplasts , Lolium latent virus , Nicotiana benthamiana , virus infection and virus-host interaction
© Microbiology Society
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2018-05-01
2024-04-19
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The interaction of Lolium latent virus major coat protein with ankyrin repeat protein NbANKr redirects it to chloroplasts and modulates virus infection
J. Gen. Virol. 99, 730 (2018); https://doi.org/10.1099/jgv.0.001043
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