RT Journal Article SR Electronic(1) A1 Bonnin, Ariane A1 Danneels, Adeline A1 Dubuisson, Jean A1 Goffard, Anne A1 Belouzard, SandrineYR 2018 T1 HCoV-229E spike protein fusion activation by trypsin-like serine proteases is mediated by proteolytic processing in the S2′ region JF Journal of General Virology, VO 99 IS 7 SP 908 OP 912 DO https://doi.org/10.1099/jgv.0.001074 PB Microbiology Society, SN 1465-2099, AB Human coronavirus 229E (HCoV-229E) is responsible for common colds. Like other coronaviruses, HCoV-229E exploits cellular proteases to activate fusion mediated by the spike protein. We analysed the proteolytic processing of the HCoV-229E spike protein by trypsin-like serine proteases leading to activation of the fusion process. Unlike in other coronaviruses, HCoV-229E fusion activation appears to be a one-step process. Indeed, cleavage of the S1/S2 interface does not seem to be a prerequisite, and the fusion activation is highly reliant on the S2′ region, with arginine residue 683 acting as the recognition site., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.001074