1887

Abstract

Rotavirus species H (RVH) has been detected in pigs, humans and bats. Moreover, porcine RVHs have often been identified in several swine-producing countries in recent years. Despite their zoonotic impact, genome information for RVHs is still limited. This study aimed to establish a tentative complete genome-based genotyping system for RVH by appending genomic sequences from 12 porcine RVHs identified in Japan between 2013 and 2015 to those from human and other porcine RVHs reported in previous studies. Phylogenetic analysis of 11 RNA segments indicated that porcine RVHs could be classified into multiple genotypes. Consequently, the genotype classification for RVHs revealed the existence of genotypes 10G, 6P, 6I, 3R, 4C, 7M, 6A, 2N, 4T, 6E and 3H for the genes VP7, VP4, VP6, VP1, VP2, VP3, NSP1, NSP2, NSP3, NSP4 and NSP5, respectively. Surprisingly, two distinctive types of NSP1 and NSP3 genes were identified from among the 12 porcine RVHs. Our data suggest a potentially novel gene recombination event between porcine RVH and rotavirus species C in the NSP3 gene. These findings would provide a new insight into the evolution of rotavirus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/jgv.0.001162
2018-10-17
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/99/12/1582.html?itemId=/content/journal/jgv/10.1099/jgv.0.001162&mimeType=html&fmt=ahah

References

  1. Estes MK, Cohen J. Rotavirus gene structure and function. Microbiol Rev 1989; 53:410–449[PubMed]
    [Google Scholar]
  2. Matthijnssens J, Otto PH, Ciarlet M, Desselberger U, van Ranst M et al. VP6-sequence-based cutoff values as a criterion for rotavirus species demarcation. Arch Virol 2012; 157:1177–1182 [View Article][PubMed]
    [Google Scholar]
  3. Mihalov-Kovács E, Gellért Á, Marton S, Farkas SL, Fehér E et al. Candidate new rotavirus species in sheltered dogs, Hungary. Emerg Infect Dis 2015; 21:660–663 [View Article][PubMed]
    [Google Scholar]
  4. Pesavento JB, Crawford SE, Estes MK, Prasad BV. Rotavirus proteins: structure and assembly. Curr Top Microbiol Immunol 2006; 309:189–219[PubMed]
    [Google Scholar]
  5. Wakuda M, Ide T, Sasaki J, Komoto S, Ishii J et al. Porcine rotavirus closely related to novel group of human rotaviruses. Emerg Infect Dis 2011; 17:1491–1493 [View Article][PubMed]
    [Google Scholar]
  6. Yang H, Makeyev EV, Kang Z, Ji S, Bamford DH et al. Cloning and sequence analysis of dsRNA segments 5, 6 and 7 of a novel non-group A, B, C adult rotavirus that caused an outbreak of gastroenteritis in China. Virus Res 2004; 106:15–26 [View Article][PubMed]
    [Google Scholar]
  7. Alam MM, Kobayashi N, Ishino M, Ahmed MS, Ahmed MU et al. Genetic analysis of an ADRV-N-like novel rotavirus strain B219 detected in a sporadic case of adult diarrhea in Bangladesh. Arch Virol 2007; 152:199–208 [View Article][PubMed]
    [Google Scholar]
  8. Jiang S, Ji S, Tang Q, Cui X, Yang H et al. Molecular characterization of a novel adult diarrhoea rotavirus strain J19 isolated in China and its significance for the evolution and origin of group B rotaviruses. J Gen Virol 2008; 89:2622–2629 [View Article][PubMed]
    [Google Scholar]
  9. Marthaler D, Rossow K, Culhane M, Goyal S, Collins J et al. Widespread rotavirus H in commercially raised pigs, United States. Emerg Infect Dis 2014; 20:1203–1206 [View Article]
    [Google Scholar]
  10. Molinari BL, Alfieri AF, Alfieri AA. Genetic variability of VP6, VP7, VP4, and NSP4 genes of porcine rotavirus group H detected in Brazil. Virus Res 2015; 197:48–53 [View Article][PubMed]
    [Google Scholar]
  11. Nyaga MM, Peenze I, Potgieter CA, Seheri LM, Page NA et al. Complete genome analyses of the first porcine rotavirus group H identified from a South African pig does not provide evidence for recent interspecies transmission events. Infect Genet Evol 2016; 38:1–7 [View Article][PubMed]
    [Google Scholar]
  12. Phan MVT, Anh PH, Cuong NV, Munnink BBO, van der Hoek L et al. Unbiased whole-genome deep sequencing of human and porcine stool samples reveals circulation of multiple groups of rotaviruses and a putative zoonotic infection. Virus Evol 2016; 2:vew027 [View Article][PubMed]
    [Google Scholar]
  13. Yinda CK, Ghogomu SM, Conceição-Neto N, Beller L, Deboutte W et al. Cameroonian fruit bats habor divergent viruses, including rotavirus H, bastroviruses, and picobirnaviruses using an alternative genetic code. Virus Evol 2018; 3:vey008
    [Google Scholar]
  14. Kobayashi N, Alam M, Kojima K, Mise K, Ishino M et al. Genomic diversity and evolution of rotaviruses: an overview. In Kobayashi N. (editor) Genomic Diversity and Molecular Epidemiology of Rotaviruses Trivandrum, India: Research Signpost; 2003 pp. 75–90
    [Google Scholar]
  15. Suzuki T, Hasebe A. A provisional complete genome-based genotyping system for rotavirus species C from terrestrial mammals. J Gen Virol 2017; 98:2647–2662 [View Article][PubMed]
    [Google Scholar]
  16. Soma J, Tsunemitsu H, Miyamoto T, Suzuki G, Sasaki T et al. Whole-genome analysis of two bovine rotavirus C strains: Shintoku and Toyama. J Gen Virol 2013; 94:128–135 [View Article][PubMed]
    [Google Scholar]
  17. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  18. Matthijnssens J, Ciarlet M, Heiman E, Arijs I, Delbeke T et al. Full genome-based classification of rotaviruses reveals a common origin between human Wa-Like and porcine rotavirus strains and human DS-1-like and bovine rotavirus strains. J Virol 2008; 82:3204–3219 [View Article][PubMed]
    [Google Scholar]
  19. Matthijnssens J, Ciarlet M, Rahman M, Attoui H, Bányai K et al. Recommendations for the classification of group A rotaviruses using all 11 genomic RNA segments. Arch Virol 2008; 153:1621–1629 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/jgv.0.001162
Loading
/content/journal/jgv/10.1099/jgv.0.001162
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error