@article{mbs:/content/journal/jgv/10.1099/jgv.0.001188, author = "Zhou, Kelei and Yue, Hua and Tang, Cheng and Ruan, Wenqiang and Zhou, Qun and Zhang, Bin", title = "Prevalence and genome characteristics of atypical porcine pestivirus in southwest China", journal= "Journal of General Virology", year = "2019", volume = "100", number = "1", pages = "84-88", doi = "https://doi.org/10.1099/jgv.0.001188", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.001188", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "atypical porcine pestivirus", keywords = "complete genome", keywords = "novel genogroup E", keywords = "congenital tremor", abstract = "Atypical porcine pestivirus (APPV) causes congenital tremor (CT) in piglets and has a wide geographical distribution. In this study, we evaluated APPV prevalence using 165 piglet sera from southwest China. Viral RNA was detectable by qRT-PCR in 43.6 % (17/39, 95 % CI 27.8–60.4 %) of piglets with CT, while viral RNA was not detected in the sera of any healthy piglets. The seven complete APPV genomes were obtained from distinct farms and were 11 269–11 459 nucleotides in length. The genomes of the seven strains shared 82.8–98 % identity with the APPV reference strains. Phylogenetic analysis of the complete genomes as well as E2 and Nrpo sequences revealed that the seven APPVs clustered into two groups: four strains belonged to genogroups A and D and three strains belonged to a novel APPV genotype, tentatively called genogroup E. This study provides important insights into the epidemiological features and genetic diversity of APPV.", }