Proteomic analysis of the Heliothis virescens ascovirus 3i (HvAV-3i) virion
Chen, Zi-Shu and Cheng, Xiao-Wen and Wang, Xing and Hou, Dian-Hai and Huang, Guo-Hua,, 100, 301-307 (2019),
doi = https://doi.org/10.1099/jgv.0.001197,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= Ascoviruses are enveloped, circular, double-stranded DNA viruses that can effectively control the appetite of lepidopteran larvae, thereby reducing the consequent damage and economic losses to crops. In this study, the virion of a sequenced Heliothis virescens ascovirus 3i (HvAV-3i) strain was used to perform proteomic analysis using both in-gel and in-solution digestion. A total of 81 viral proteins, of which 67 were associated with the virions, were identified in the proteome of HvAV-3i virions. Among these proteins, 23 with annotated functions were associated with DNA/RNA metabolism/transcription, virion assembly, sugar and lipid metabolism, signalling, cellular homoeostasis and cell lysis. Twenty-one viral membrane proteins were also identified. Some of the minor ‘virion’ proteins identified may be non-virion contaminants of viral proteins synthesized during replication, identified by more recent and highly sensitive methods. The extensive identification of the ascoviral proteome will establish a foundation for further investigation of ascoviral replication and infection.,
language=,
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