1887

Abstract

Stretch Lagoon orbivirus (SLOV) was isolated in 2002 from pooled mosquitoes collected at Stretch Lagoon, near the Wolfe Creek national park in the Kimberley region of Western Australia. Conventional serological tests were unable to identify the isolate, and electron microscopy indicated a virus of the genus , family . Here, a cDNA subtraction method was used to obtain approximately one-third of the viral genome, and further sequencing was performed to complete the sequences of segment 1 (viral polymerase) and segment 2 (conserved inner-core protein). Phylogenetic analysis showed that SLOV should be considered a new species within the genus . A real-time RT-PCR test was designed to study the epidemiology of SLOV in the field. Six additional isolates of SLOV were identified, including isolates from four additional locations and two additional mosquito species. Horses, donkeys and goats were implicated as potential vertebrate hosts in a serological survey.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.010074-0
2009-06-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/90/6/1433.html?itemId=/content/journal/jgv/10.1099/vir.0.010074-0&mimeType=html&fmt=ahah

References

  1. Attoui H., Stirling J. M., Munderloh U. G., Billoir F., Brookes S. M., Burroughs J. N., de Micco P., Mertens P. P., de Lamballerie X. 2001; Complete sequence characterization of the genome of the St Croix River virus, a new orbivirus isolated from cells of Ixodes scapularis . J Gen Virol 82:795–804
    [Google Scholar]
  2. Attoui H., Mohd Jaafar F., Belhouchet M., Aldrovandi N., Tao S., Chen B., Liang G., Tesh R. B., de Micco P., de Lamballerie X. 2005; Yunnan orbivirus , a new orbivirus species isolated from Culex tritaeniorhynchus mosquitoes in China. J Gen Virol 86:3409–3417 [CrossRef]
    [Google Scholar]
  3. Broom A. K., Wright A. E., MacKenzie J. S., Lindsay M. D., Robinson D. 1989; Isolation of Murray Valley encephalitis and Ross River viruses from Aedes normanensis (Diptera: Culicidae) in Western Australia. J Med Entomol 26:100–103 [CrossRef]
    [Google Scholar]
  4. Broom A. K., Hall R. A., Johansen C. A., Oliveira N., Howard M. A., Lindsay M. D., Kay B. H., Mackenzie J. S. 1998; Identification of Australian arboviruses in inoculated cell cultures using monoclonal antibodies in ELISA. Pathology 30:286–288 [CrossRef]
    [Google Scholar]
  5. Cowled C., Melville L., Weir R., Walsh S., Hyatt A., Van Driel R., Davis S., Gubala A., Boyle D. 2007; Genetic and epidemiological characterization of Middle Point orbivirus, a novel virus isolated from sentinel cattle in northern Australia. J Gen Virol 88:3413–3422 [CrossRef]
    [Google Scholar]
  6. Diatchenko L., Lau Y. F., Campbell A. P., Chenchik A., Moqadam F., Huang B., Lukyanov S., Lukyanov K., Gurskaya N. other authors 1996; Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proc Natl Acad Sci U S A 93:6025–6030 [CrossRef]
    [Google Scholar]
  7. Johansen C. A., van den Hurk A. F., Ritchie S. A., Zborowski P., Nisbet D. J., Paru R., Bockarie M. J., Macdonald J., Drew A. C. other authors 2000; Isolation of Japanese encephalitis virus from mosquitoes (Diptera: Culicidae) collected in the Western Province of Papua New Guinea, 1997–1998. Am J Trop Med Hyg 62:631–638
    [Google Scholar]
  8. Kozak M. 1997; Recognition of AUG and alternative initiator codons is augmented by G in position +4 but is not generally affected by the nucleotides in positions +5 and +6. EMBO J 16:2482–2492 [CrossRef]
    [Google Scholar]
  9. Lindsay M. D., Broom A. K., Wright A. E., Johansen C. A., Mackenzie J. S. 1993; Ross River virus isolations from mosquitoes in arid regions of Western Australia: implication of vertical transmission as a means of persistence of the virus. Am J Trop Med Hyg 49:686–696
    [Google Scholar]
  10. Mandl C. W., Heinz F. X., Puchhammer-Stockl E., Kunz C. 1991; Sequencing the termini of capped viral RNA by 5′-3′ ligation and PCR. Biotechniques 10:484–486
    [Google Scholar]
  11. Mertens P. P. C., Maan S., Samuel A., Attoui H. 2005; Genus Orbivirus. In Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses pp 466–483Edited by Fauquet C. M., Mayo M. A., Maniloff J., Desselberger U., Ball. London: Elsevier/Academic Press;
    [Google Scholar]
  12. National Arbovirus Monitoring Program; 2008; National Arbovirus Monitoring Program Annual Report 2007–2008. Animal Health Australia: http://www.animalhealthaustralia.com.au/
  13. Page R. D. 1996; TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358
    [Google Scholar]
  14. Rost B., Yachdav G., Liu J. 2004; The PredictProtein server. Nucleic Acids Res 32:W321–W326 [CrossRef]
    [Google Scholar]
  15. St George T. D. 1982; Veterinary arboviruses – across Wallace's line. In Arbovirus Research in Australia – Proceedings of the 6th Symposium Brisbane Australia pp 279–282 Rockhampton: CSIRO Division of Tropical Animal Production;
    [Google Scholar]
  16. St George T. D., Cybinski D. H., Main A. J., McKilligan N., Kemp D. H. 1984; Isolation of a new arbovirus from the tick Argas robertsi from a cattle egret ( Bubulcus ibis coromandus ) colony in Australia. Aust J Biol Sci 37:85–89
    [Google Scholar]
  17. St George T. D., Baldock C., Bellis G., Bishop A., Cameron A., Doherty B., Ellis T., Gard G., Johnson S. other authors 2001; The history of bluetongue, akabane and ephemeral fever viruses and their vectors in Australia 1975–1999. Animal Health Australia: http://www.animalhealthaustralia.com.au/
  18. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  19. Weir R. P., Hyatt A. D., Calisher H. H., Whelan P. I. 1997; New records of arboviruses isolated from mosquitoes in the Northern Territory, 1982–1992. Arbovirus Res Aust 7:311–321
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.010074-0
Loading
/content/journal/jgv/10.1099/vir.0.010074-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error