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Volume 90, Issue 9

Full-length sequencing and genetic characterization of Breu Branco virus (, ) and two related strains isolated from mosquitoes Free

Abstract

Breu Branco virus (BE AR 492347) was isolated from () mosquitoes captured in Tucuruí, Pará State, northern Brazil, in 1988. No cross-reactivity by complement-fixation tests was observed between Breu Branco virus and other known arboviruses. Results of electron microscopy and physicochemical tests suggested that Breu Branco virus may be a member of the family . In order to elucidate its taxonomic status, a comprehensive genetic characterization was conducted for Breu Branco virus and related strains (BE AR 494475 and BE AR 486204) that were also isolated from mosquitoes in the same area. This included full-length genome sequencing, determination of genetic traits and phylogenetic analysis. Breu Branco virus showed a similar genome organization to members of the genus , family . Genetically, Breu Branco virus was indistinguishable from strains BE AR 494475 and BE AR 486204. Phylogenetic analysis suggested that Breu Branco virus BE AR 492347 and its related strains constitute a novel species of the genus . Breu Branco virus is the first Brazilian orbivirus and the fifth orbivirus in the world to be sequenced fully.

  • Received:
  • Accepted:
  • Published Online:
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2009-09-01
2024-04-20
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References

  1. Attoui, H., Billoir, F., Biagini, P., Micco, P. & Lamballerie, X.(2000a). Complete sequence determination and genetic analysis of Banna virus and Kadipiro virus: proposal for assignment to a new genus (Seadornavirus) within the family Reoviridae. J Gen Virol 81, 1507–1515. [Google Scholar]
  2. Attoui, H., Billoir, F., Cantaloube, J. F., Biagini, P., Micco, P. & Lamballerie, X.(2000b). Strategies for the sequence determination of viral dsRNA genomes. J Virol Methods 89, 147–158.[CrossRef] [Google Scholar]
  3. Attoui, H., Stirling, J. M., Munderloh, U. G., Billoir, F., Brookes, S. M., Burroughs, J. N., Micco, P., Mertens, P. P. C. & Lamballerie, X.(2001). Complete sequence characterization of genome of St Croix River virus, a new orbivirus isolated from cells of Ixodes scapularis. J Gen Virol 82, 795–804. [Google Scholar]
  4. Attoui, H., Mohd Jaafar, F., Belhouchet, M., Aldrovandi, N., Tao, S., Chen, B., Liang, G., Tesh, R. B., de Micco, P. & de Lamballerie, X.(2005).Yunnan orbivirus, a new orbivirus species isolated from Culex tritaeniorhynchus mosquitoes in China. J Gen Virol 86, 3409–3417.[CrossRef] [Google Scholar]
  5. Bryant, J. E., Vasconcelos, P. F., Rijnbrand, R. C., Mutebi, J. P., Higgs, S. & Barrett, A. D.(2005). Size heterogeneity in the 3′ noncoding region of South American isolates of yellow fever virus. J Virol 79, 3807–3821.[CrossRef] [Google Scholar]
  6. Chaignat, V., Worwa, G., Scherrer, N., Hilbe, M., Ehrensperger, F., Batten, C., Cortyen, M., Hofmann, M. & Thuer, B.(2009). Toggenburg orbivirus, a new bluetongue virus: initial detection, first observations in field and experimental infection of goats and sheep. Vet Microbiol (in press). doi:10.1016/j.vetmic.2009.02.003 [Google Scholar]
  7. Cowled, C., Melville, L., Weir, R., Walsh, S., Hyatt, A., Van Driel, R., Davis, S., Gubala, A. & Boyle, D.(2007). Genetic and epidemiological characterization of Middle Point orbivirus, a novel virus isolated from sentinel cattle in northern Australia. J Gen Virol 88, 3413–3422.[CrossRef] [Google Scholar]
  8. Cowled, C., Palacios, G., Melville, L., Weir, R., Walsh, S., Davis, S., Gubala, A., Lipkin, W. I., Briese, T. & Boyle, D.(2009). Genetic and epidemiological characterization of Stretch Lagoon orbivirus, a novel orbivirus species isolated from Culex and Aedes mosquitoes in northern Australia. J Gen Virol 90, 1433–1439.[CrossRef] [Google Scholar]
  9. Efron, B.(1982).The Jackknife, the Bootstrap and Other Resampling Plans (CBMS-NSF Regional Conference Series in Applied Mathematics, Monograph 38). Philadelphia, PA: Society for Industrial and Applied Mathematics.
  10. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  11. Kar, A. K. & Roy, P.(2003). Defining the structure-function relationships of bluetongue virus helicase protein VP6. J Virol 77, 11347–11356.[CrossRef] [Google Scholar]
  12. Kimura, M.(1980). A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  13. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  14. Mertens, P. P. C., Duncan, R., Attoui, H. & Dermody, T. S.(2005). Family Reoviridae. In Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses, pp. 447–454. Edited by C. M. Fauquet, M. A. Mayo, J. Maniloff, U. Desselberg & L. A. Ball. San Diego, CA: Elsevier Academic Press.
  15. Moss, S. R., Jones, L. D. & Nuttall, P. A.(1992). Comparison of the major structural core proteins of tick-borne and Culicoides-borne orbiviruses. J Gen Virol 73, 2585–2590.[CrossRef] [Google Scholar]
  16. Parkes, H. & Gould, A. R.(1996). Characterisation of Wongorr virus, an Australian orbivirus. Virus Res 44, 111–122.[CrossRef] [Google Scholar]
  17. Purse, B. V., Mellor, P. S., Rogers, D. J., Samuel, A. R., Mertens, P. P. & Baylis, M.(2005). Climate change and the recent emergence of bluetongue in Europe. Nat Rev Microbiol 3, 171–181.[CrossRef] [Google Scholar]
  18. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstruction phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  19. Shapiro, A. L., Viñuela, E. & Maizel, J. V., Jr(1967). Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem Biophys Res Commun 28, 815–820.[CrossRef] [Google Scholar]
  20. Stäuber, N., Martinez-Costas, J., Sutton, G., Monastyrskaya, K. & Roy, P.(1997). Bluetongue virus VP6 protein binds ATP and exhibits an RNA-dependent ATPase function and a helicase activity that catalyze the unwinding of double-stranded RNA substrates. J Virol 71, 7220–7226. [Google Scholar]
  21. Swofford, D. L.(1999).paup*: phylogenetic analysis using parsimony (*and other methods), version 4. Sunderland, MA: Sinauer Associates.
  22. Theiler, M.(1957). Action of sodium desoxycholate on arthropod-borne viruses. Proc Soc Exp Biol Med 96, 380–382.[CrossRef] [Google Scholar]
  23. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  24. Travassos da Rosa, A. P. A., Tesh, R. B., Pinheiro, F. P., Travassos da Rosa, J. F., Peralta, P. H. & Knudson, D. L.(1984). Characterization of the Changuinola serogroup viruses (Reoviridae: Orbivirus). Intervirology 21, 38–49.[CrossRef] [Google Scholar]
  25. Travassos da Rosa, J. F., Travassos da Rosa, A. P. A., Dégallier, N. & Vasconcelos, P. F. C.(1992). Caracterização e relacionamento antigênico de três novos Bunyavirus do grupo Anopheles A (Bunyaviridae) dos arbovírus. Revista de Saúde Pública 26, 173–178 (in Portuguese).[CrossRef] [Google Scholar]
  26. Vasconcelos, P. F., Bryant, J. E., Travassos da Rosa, A. P. A., Tesh, R. B., Rodrigues, S. G. & Barrett, A. D.(2004). Genetic divergence and dispersal of yellow fever virus, Brazil. Emerg Infect Dis 10, 1578–1584.[CrossRef] [Google Scholar]
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Full-length sequencing and genetic characterization of Breu Branco virus (Reoviridae, Orbivirus) and two related strains isolated from Anopheles mosquitoes
J. Gen. Virol. 90, 2183 (2009); https://doi.org/10.1099/vir.0.010165-0
/content/journal/jgv/10.1099/vir.0.010165-0
/content/journal/jgv/10.1099/vir.0.010165-0
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Supplements

vol. , part 9, pp. 2183 – 2190

Specific primers used for full-length sequencing of the Breu Branco, BE AR 494475 and BE AR 486204 virus genomes.

Selected orbiviruses and other members of the family used for phylogenetic analysis.

Genetic relationships among Breu Branco virus and strains BE AR 494475 and BE AR 486204 by each genome segment.

Summary of the nucleotide and amino acid substitutions along the entire S9 ORF of Breu Branco, BE AR 494475 and BE AR 486204 viruses.

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