RT Journal Article SR Electronic(1) A1 Rosario, Karyna A1 Marinov, Milen A1 Stainton, Daisy A1 Kraberger, Simona A1 Wiltshire, Elizabeth J. A1 Collings, David A. A1 Walters, Matthew A1 Martin, Darren P. A1 Breitbart, Mya A1 Varsani, ArvindYR 2011 T1 Dragonfly cyclovirus, a novel single-stranded DNA virus discovered in dragonflies (Odonata: Anisoptera) JF Journal of General Virology, VO 92 IS 6 SP 1302 OP 1308 DO https://doi.org/10.1099/vir.0.030338-0 PB Microbiology Society, SN 1465-2099, AB Dragonfly cyclovirus (DfCyV), a new species of ssDNA virus discovered using viral metagenomics in dragonflies (familyLibellulidae) from the Kingdom of Tonga. Metagenomic sequences of DfCyV were similar to viruses of the recently proposed genus Cyclovirus within the family Circoviridae. Specific PCRs resulted in the recovery of 21 DfCyV genomes from three dragonfly species (Pantala flavescens, Tholymis tillarga and Diplacodes bipunctata). The 1741 nt DfCyV genomes share >95 % nucleotide identity and are classified into 11 subtypes representing a single strain. The DfCyV genomes share 48–63 % genome-wide nucleotide identity with cycloviruses identified in human faecal samples. Recombination analysis revealed three recombinant DfCyV genomes, suggesting that recombination plays an important role in cyclovirus evolution. To our knowledge, this is the first report of a circular ssDNA virus identified in insects, and the data may help elucidate evolutionary links among novel Circoviridae recently identified in animals and environmental samples., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.030338-0