Cyclosporin A inhibits the replication of diverse coronaviruses
de Wilde, Adriaan H. and Zevenhoven-Dobbe, Jessika C. and van der Meer, Yvonne and Thiel, Volker and Narayanan, Krishna and Makino, Shinji and Snijder, Eric J. and van Hemert, Martijn J.,, 92, 2542-2548 (2011),
doi = https://doi.org/10.1099/vir.0.034983-0,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= Low micromolar, non-cytotoxic concentrations of cyclosporin A (CsA) strongly affected the replication of severe acute respiratory syndrome coronavirus (SARS-CoV), human coronavirus 229E and mouse hepatitis virus in cell culture, as was evident from the strong inhibition of GFP reporter gene expression and a reduction of up to 4 logs in progeny titres. Upon high-multiplicity infection, CsA treatment rendered SARS-CoV RNA and protein synthesis almost undetectable, suggesting an early block in replication. siRNA-mediated knockdown of the expression of the prominent CsA targets cyclophilin A and B did not affect SARS-CoV replication, suggesting either that these specific cyclophilin family members are dispensable or that the reduced expression levels suffice to support replication.,
language=,
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