RT Journal Article SR Electronic(1) A1 de Wilde, Adriaan H. A1 Zevenhoven-Dobbe, Jessika C. A1 van der Meer, Yvonne A1 Thiel, Volker A1 Narayanan, Krishna A1 Makino, Shinji A1 Snijder, Eric J. A1 van Hemert, Martijn J.YR 2011 T1 Cyclosporin A inhibits the replication of diverse coronaviruses JF Journal of General Virology, VO 92 IS 11 SP 2542 OP 2548 DO https://doi.org/10.1099/vir.0.034983-0 PB Microbiology Society, SN 1465-2099, AB Low micromolar, non-cytotoxic concentrations of cyclosporin A (CsA) strongly affected the replication of severe acute respiratory syndrome coronavirus (SARS-CoV), human coronavirus 229E and mouse hepatitis virus in cell culture, as was evident from the strong inhibition of GFP reporter gene expression and a reduction of up to 4 logs in progeny titres. Upon high-multiplicity infection, CsA treatment rendered SARS-CoV RNA and protein synthesis almost undetectable, suggesting an early block in replication. siRNA-mediated knockdown of the expression of the prominent CsA targets cyclophilin A and B did not affect SARS-CoV replication, suggesting either that these specific cyclophilin family members are dispensable or that the reduced expression levels suffice to support replication., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.034983-0