@article{mbs:/content/journal/jgv/10.1099/vir.0.047860-0, author = "Ishikawa, Kazuya and Maejima, Kensaku and Komatsu, Ken and Netsu, Osamu and Keima, Takuya and Shiraishi, Takuya and Okano, Yukari and Hashimoto, Masayoshi and Yamaji, Yasuyuki and Namba, Shigetou", title = "Fig mosaic emaravirus p4 protein is involved in cell-to-cell movement", journal= "Journal of General Virology", year = "2013", volume = "94", number = "3", pages = "682-686", doi = "https://doi.org/10.1099/vir.0.047860-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.047860-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = " Fig mosaic virus (FMV), a member of the newly formed genus Emaravirus, is a segmented negative-strand RNA virus. Each of the six genomic FMV segments contains a single ORF: that of RNA4 encodes the protein p4. FMV-p4 is presumed to be the movement protein (MP) of the virus; however, direct experimental evidence for this is lacking. We assessed the intercellular distribution of FMV-p4 in plant cells by confocal laser scanning microscopy and we found that FMV-p4 was localized to plasmodesmata and to the plasma membrane accompanied by tubule-like structures. A series of experiments designed to examine the movement functions revealed that FMV-p4 has the capacity to complement viral cell-to-cell movement, prompt GFP diffusion between cells, and spread by itself to neighbouring cells. Altogether, our findings demonstrated that FMV-p4 shares several properties with other viral MPs and plays an important role in cell-to-cell movement.", }