@article{mbs:/content/journal/jgv/10.1099/vir.0.057299-0, author = "Di Pilato, Mauro and Mejías-Pérez, Ernesto and Gómez, Carmen Elena and Perdiguero, Beatriz and Sorzano, Carlos Oscar S. and Esteban, Mariano", title = "New vaccinia virus promoter as a potential candidate for future vaccines", journal= "Journal of General Virology", year = "2013", volume = "94", number = "12", pages = "2771-2776", doi = "https://doi.org/10.1099/vir.0.057299-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.057299-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Here we describe the design and strength of a new synthetic late-early optimized (LEO) vaccinia virus (VACV) promoter used as a transcriptional regulator of GFP expression during modified vaccinia Ankara infection. In contrast to the described synthetic VACV promoter (pS), LEO induced significantly higher levels of GFP expression in vitro within the first hour after infection, which correlated with an enhancement in the GFP-specific CD8 T-cell response detected in vivo, demonstrating its potential use in future vaccines.", }