@article{mbs:/content/journal/jgv/10.1099/vir.0.068700-0, author = "Bodewes, Rogier and Lempp, Charlotte and Schürch, Anita C. and Habierski, Andre and Hahn, Kerstin and Lamers, Mart and von Dörnberg, Katja and Wohlsein, Peter and Drexler, Jan Felix and Haagmans, Bart L. and Smits, Saskia L. and Baumgärtner, Wolfgang and Osterhaus, Albert D. M. E.", title = "Novel divergent nidovirus in a python with pneumonia", journal= "Journal of General Virology", year = "2014", volume = "95", number = "11", pages = "2480-2485", doi = "https://doi.org/10.1099/vir.0.068700-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.068700-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "The order Nidovirales contains large, enveloped viruses with a non-segmented positive-stranded RNA genome. Nidoviruses have been detected in man and various animal species, but, to date, there have been no reports of nidovirus in reptiles. In the present study, we describe the detection, characterization, phylogenetic analyses and disease association of a novel divergent nidovirus in the lung of an Indian python (Python molurus) with necrotizing pneumonia. Characterization of the partial genome (>33 000 nt) of this virus revealed several genetic features that are distinct from other nidoviruses, including a very large polyprotein 1a, a putative ribosomal frameshift signal that was identical to the frameshift signal of astroviruses and retroviruses and an accessory ORF that showed some similarity with the haemagglutinin–neuraminidase of paramyxoviruses. Analysis of genome organization and phylogenetic analysis of polyprotein 1ab suggests that this virus belongs to the subfamily Torovirinae. Results of this study provide novel insights into the genetic diversity within the order Nidovirales.", }