1887

Abstract

Replication of tobacco mosaic virus (TMV) is connected with endoplasmic reticulum (ER)-associated membranes at early stages of infection. This study reports that TMV movement protein (MP)-specific protein kinases (PKs) associated with the ER of tobacco were capable of phosphorylating Thr in TMV MP. The MP-specific PKs with apparent molecular masses of about 45–50 kDa and 38 kDa were revealed by gel PK assays. Two types of mutations were introduced in TMV MP gene of wild-type TMV U1 genome to substitute Thr by neutral Ala or by negatively charged Asp. Mutation of Thr to Ala did not affect the size of necrotic lesions induced by the mutant virus in Xanthi nc. plants. Conversely, mutation of Thr to Asp mimicking Thr phosphorylation strongly inhibited cell-to-cell movement. The possible role of Thr phosphorylation in TMV MP function is discussed.

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2003-03-01
2024-04-25
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References

  1. Atkins D., Hull R., Wells B., Roberts K., Moore P., Beachy R. N. 1991; The tobacco mosaic virus 30K movement protein in transgenic tobacco plants is localized to plasmodesmata. J Gen Virol 72:209–211
    [Google Scholar]
  2. Boyko V., Ashby J. A., Suslova E., Ferralli J., Sterthaus O., Deom C. M., Heinlein M. 2002; Intramolecular complementing mutations in tobacco mosaic virus movement protein confirm a role for microtubule association in viral RNA transport. J Virol 76:3974–3980
    [Google Scholar]
  3. Carrington J. C., Kasschau K. D., Mahajan S. K., Schaad M. C. 1996; Cell-to-cell and long-distance transport of viruses in plants. Plant Cell 8:1669–1681
    [Google Scholar]
  4. Citovsky V., McLean B. G., Zupan J. R., Zambryski P. 1993; Phosphorylation of tobacco mosaic virus cell-to-cell movement protein by a developmentally regulated plant cell wall-associated protein kinase. Genes Dev 7:904–910
    [Google Scholar]
  5. Deom C. M., He X. Z. 1997; Second-site reversion of a dysfunctional mutation in a conserved region of the tobacco mosaic tobamovirus movement protein. Virology 232:13–18
    [Google Scholar]
  6. Ding B., Haudenshield J. S., Hull R. J., Wolf S., Beachy R. N., Lucas W. J. 1992; Secondary plasmodesmata are specific sites of localization of the tobacco mosaic virus movement protein in transgenic tobacco plants. Plant Cell 4:915–928
    [Google Scholar]
  7. Haley A., Hunter T., Kiberstis P., Zimmern D. 1995; Multiple serine phosphorylation sites on the 30 kDa TMV cell-to-cell movement protein synthesized in tobacco protoplasts. Plant J 8:715–724
    [Google Scholar]
  8. Heinlein M., Epel B. L., Padgett H. S., Beachy R. N. 1995; Interaction of tobamovirus movement proteins with the plant cytoskeleton. Science 270:1983–1985
    [Google Scholar]
  9. Heinlein M., Padgett H. S., Gens J. S., Pickard B. G., Casper S. J., Epel B. L., Beachy R. N. 1998; Changing patterns of localization of the tobacco mosaic virus movement protein and replicase to the endoplasmic reticulum and microtubules during infection. Plant Cell 10:1107–1120
    [Google Scholar]
  10. Karpova O. V., Ivanov K. I., Rodionova N. P., Dorokhov Yu. L., Atabekov J. G. 1997; Nontranslatability and dissimilar behavior in plants and protoplasts of viral RNA and movement protein complexes formed in vitro . Virology 230:11–21
    [Google Scholar]
  11. Karpova O. V., Rodionova N. P., Ivanov K. I., Kozlovsky S. V., Dorokhov Y. L., Atabekov J. G. 1999; Phosphorylation of tobacco mosaic virus movement protein abolishes its translation repressing ability. Virology 261:20–24
    [Google Scholar]
  12. Kawakami S., Padgett H. S., Hosokawa D., Okada Y., Beachy R. N., Watanabe Y. 1999; Phosphorylation and/or presence of serine 37 in the movement protein of tomato mosaic tobamovirus is essential for intracellular localization and stability in vivo . J Virol 73:6831–6840
    [Google Scholar]
  13. Koonin E. V., Mushegian A. R., Ryabov E. V., Dolja V. V. 1991; Diverse groups of plant RNA and DNA viruses share related movement proteins that may possess chaperone-like activity. J Gen Virol 72:2895–2903
    [Google Scholar]
  14. Lazarowitz S. G., Beachy R. N. 1999; Viral movement proteins as probes for intracellular and intercellular trafficking in plants. Plant Cell 11:535–548
    [Google Scholar]
  15. Lee J. Y., Lucas W. J. 2001; Phosphorylation of viral movement proteins: regulation of cell-to-cell trafficking. Trends Microbiol 9:5–8
    [Google Scholar]
  16. Mas P., Beachy R. N. 1999; Replication of tobacco mosaic virus on endoplasmic reticulum and role of the cytoskeleton and virus movement protein in intracellular distribution of viral RNA. J Cell Biol 147:945–958
    [Google Scholar]
  17. Matsushita Y., Hanazawa K., Yoshioka K., Oguchi T., Kawakami S., Watanabe Y., Nishiguchi M., Nyunoya H. 2000; In vitro phosphorylation of the movement protein of tomato mosaic tobamovirus by a cellular kinase. J Gen Virol 81:2095–2102
    [Google Scholar]
  18. McLean B. G., Zupan J., Zambryski P. C. 1995; Tobacco mosaic virus movement protein associates with the cytoskeleton in tobacco cells. Plant Cell 7:2101–2114
    [Google Scholar]
  19. Oparka K. J., Prior D. A., Santa Cruz S., Padgett H. S., Beachy R. N. 1997; Gating of epidermal plasmodesmata is restricted to the leading edge of expanding infection sites of tobacco mosaic virus (TMV). Plant J 12:781–789
    [Google Scholar]
  20. Reichel C., Beachy R. N. 1998; Tobacco mosaic virus infection induces severe morphological changes of the endoplasmic reticulum. Proc Natl Acad Sci U S A 95:11169–11174
    [Google Scholar]
  21. Tomenius K., Clapham D., Meshi T. 1987; Localization by immunogold cytochemistry of the virus-coded 30 K protein I plasmodesmata of leaves infected with tobacco mosaic virus. Virology 160:363–371
    [Google Scholar]
  22. Tzfira T., Rhee Y., Chen M. H., Kunik T., Citovsky V. 2000; Nucleic acid transport in plant–microbe interactions: the molecules that walk through the walls. Annu Rev Microbiol 54:187–219
    [Google Scholar]
  23. Waigmann E., Chen M. H., Bachmaier R., Ghoshroy S., Citovsky V. 2000; Regulation of plasmodesmal transport by phosphorylation of tobacco mosaic virus cell-to-cell movement protein. EMBO J 19:4875–4884
    [Google Scholar]
  24. Watanabe Y., Ogawa T., Okada Y. 1992; In vivo phosphorylation of the 30-Kda protein of tobacco mosaic virus. FEBS Lett 313:181–184
    [Google Scholar]
  25. Wilson C., Anglmayer R., Vicente O., Heberle-Bors E. 1995; Molecular cloning, functional expression in Escherichia coli , and characterization of multiple mitogen-activated-protein kinases from tobacco. Eur J Biochem 233:249–257
    [Google Scholar]
  26. Wolf S., Deom C. M., Beachy R., Lucas W. J. 1991; Plasmodesmatal function is probed using transgenic tobacco plants that express a virus movement protein. Plant Cell 3:593–604
    [Google Scholar]
  27. Zhang S., Klessig D. F. 1997; Salicylic acid activates a 48-kD MAP kinase in tobacco. Plant Cell 9:809–824
    [Google Scholar]
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