1887

Abstract

We have examined the subcellular localization properties of human adenovirus 2 (HAdV-2) preMu and mature Mu (pX) proteins as fusions with enhanced green fluorescence protein (EGFP). We determined that preMu is exclusively a nucleolar protein with a single nucleolar accumulation signal within the Mu sequence. In addition, we noted that both preMu–EGFP and Mu–EGFP are excluded from adenovirus DNA-binding protein (DBP)-rich replication centres in adenovirus-infected cells. Surprisingly, we observed that cells in which preMu–EGFP (but not Mu–EGFP) is transiently expressed prior to or shortly after infection with Ad2 did not express late adenovirus genes. Further investigation suggested this might be due to a failure to express pre-terminal protein (preTP) from the E2 region, despite expression of another E2 protein, DBP. Deletion mutagenesis identified a highly conserved region in the C terminus of preMu responsible for these observations. Thus our data suggest that preMu may play a role in modulating accumulation of proteins from the E2 region.

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2004-01-01
2024-03-29
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References

  1. Andersen J. S., Lyon C. E., Fox A. H., Leung A. K., Lam Y. W., Steen H., Mann M., Lamond A. I. 2002; Directed proteomic analysis of the human nucleolus. Curr Biol 12:1–11
    [Google Scholar]
  2. Anderson C. W., Young M. E., Flint S. J. 1989; Characterization of the adenovirus 2 virion protein, mu. Virology 172:506–512
    [Google Scholar]
  3. Askham J. M., Moncur P., Markham A. F., Morrison E. E. 2000; Regulation and function of the interaction between the APC tumour suppressor protein and EB1. Oncogene 19:1950–1958
    [Google Scholar]
  4. Boisvert F. M., Hendzel M. J., Bazett-Jones D. P. 2000; Promyelocytic leukemia (PML) nuclear bodies are protein structures that do not accumulate RNA. J Cell Biol 148:283–292
    [Google Scholar]
  5. Bridge E., Carmo-Fonseca M., Lamond A., Pettersson U. 1993; Nuclear organization of splicing small nuclear ribonucleoproteins in adenovirus-infected cells. J Virol 67:5792–5802
    [Google Scholar]
  6. Bridge E., Xia D. X., Carmo-Fonseca M., Cardinali B., Lamond A. I., Pettersson U. 1995; Dynamic organization of splicing factors in adenovirus-infected cells. J Virol 69:281–290
    [Google Scholar]
  7. Castiglia C. L., Flint S. J. 1983; Effects of adenovirus infection on rRNA synthesis and maturation in HeLa cells. Mol Cell Biol 3:662–671
    [Google Scholar]
  8. Chatterjee P. K., Vayda M. E., Flint S. J. 1985; Interactions among the three adenovirus core proteins. J Virol 55:379–386
    [Google Scholar]
  9. Davison A. J., Benkő M., Harrach B. 2003; Genetic content and evolution of adenoviruses. J Gen Virol 84:2895–2908
    [Google Scholar]
  10. Jarrous N., Wolenski J. S., Wesolowski D., Lee C., Altman S. 1999; Localization in the nucleolus and coiled bodies of protein subunits of the ribonucleoprotein ribonuclease P. J Cell Biol 146:559–572
    [Google Scholar]
  11. Liu J. L., Lee L. F., Ye Y., Qian Z., Kung H. J. 1997; Nucleolar and nuclear localization properties of a herpesvirus bZIP oncoprotein. MEQ. J Virol 71:3188–3196
    [Google Scholar]
  12. Lunt R., Vayda M. E., Young M., Flint S. J. 1988; Isolation and characterization of monoclonal antibodies against the adenovirus core proteins. Virology 164:275–279
    [Google Scholar]
  13. Matthews D. A. 2001; Adenovirus protein V induces redistribution of nucleolin and B23 from nucleolus to cytoplasm. J Virol 75:1031–1038
    [Google Scholar]
  14. Matthews D. A., Russell W. C. 1994; Adenovirus protein–protein interactions: hexon and protein VI. J Gen Virol 75:3365–3374
    [Google Scholar]
  15. Matthews D. A., Russell W. C. 1998a; Adenovirus core protein V interacts with p32 – a protein which is associated with both the mitochondria and the nucleus. J Gen Virol 79:1677–1685
    [Google Scholar]
  16. Matthews D. A., Russell W. C. 1998b; Adenovirus core protein V is delivered by the invading virus to the nucleus of the infected cell and later in infection is associated with nucleoli. J Gen Virol 79:1671–1675
    [Google Scholar]
  17. Molin M., Akusjarvi G. 2000; Overexpression of essential splicing factor ASF/SF2 blocks the temporal shift in adenovirus pre-mRNA splicing and reduces virus progeny formation. J Virol 74:9002–9009
    [Google Scholar]
  18. Molin M., Bouakaz L., Berenjian S., Akusjarvi G. 2002; Unscheduled expression of capsid protein IIIa results in defects in adenovirus major late mRNA and protein expression. Virus Res 83:197–206
    [Google Scholar]
  19. Murray K. D., Etheridge C. J., Shah S. I., Matthews D. A., Russell W., Gurling H. M., Miller A. D. 2001; Enhanced cationic liposome-mediated transfection using the DNA-binding peptide mu (mu) from the adenovirus core. Gene Ther 8:453–460
    [Google Scholar]
  20. Okuwaki M., Iwamatsu A., Tsujimoto M., Nagata K. 2001; Identification of nucleophosmin/B23, an acidic nucleolar protein, as a stimulatory factor for in vitro replication of adenovirus DNA complexed with viral basic core proteins. J Mol Biol 311:41–55
    [Google Scholar]
  21. Pederson T. 1998; The plurifunctional nucleolus. Nucleic Acids Res 26:3871–3876
    [Google Scholar]
  22. Perlaky L., Valdez B. C., Busch H. 1997; Effects of cytotoxic drugs on translocation of nucleolar RNA helicase RH-II/Gu. Exp Cell Res 235:413–420
    [Google Scholar]
  23. Pombo A., Ferreira J., Bridge E., Carmo-Fonseca M. 1994; Adenovirus replication and transcription sites are spatially separated in the nucleus of infected cells. EMBO J 13:5075–5085
    [Google Scholar]
  24. Puvion-Dutilleul F., Christensen M. E. 1993; Alterations of fibrillarin distribution and nucleolar ultrastructure induced by adenovirus infection. Eur J Cell Biol 61:168–176
    [Google Scholar]
  25. Russell W. C., Webster A., Leith I. R., Kemp G. D. 1989; Phosphorylation of adenovirus DNA-binding protein. J Gen Virol 70:3249–3259
    [Google Scholar]
  26. Scheer U., Hock R. 1999; Structure and function of the nucleolus. Curr Opin Cell Biol 11:385–390
    [Google Scholar]
  27. Shenk T. 2001; Adenoviridae : the viruses and their replication. In Fields Virology , 4th edn. pp  2265–2299 Edited by Knipe D. M., Howley P. M. Philadelphia: Lippincott Williams & Wilkins;
    [Google Scholar]
  28. Sleeman J., Lyon C. E., Platani M., Kreivi J. P., Lamond A. I. 1998; Dynamic interactions between splicing snRNPs, coiled bodies and nucleoli revealed using snRNP protein fusions to the green fluorescent protein. Exp Cell Res 243:290–304
    [Google Scholar]
  29. van Eenennaam H., van der Heijden A., Janssen R. J., van Venrooij W. J., Pruijn G. J. 2001; Basic domains target protein subunits of the RNase MRP complex to the nucleolus independently of complex association. Mol Biol Cell 12:3680–3689
    [Google Scholar]
  30. Vayda M. E., Rogers A. E., Flint S. J. 1983; The structure of nucleoprotein cores released from adenovirions. Nucleic Acids Res 11:441–460
    [Google Scholar]
  31. Webster A., Leith I. R., Nicholson J., Hounsell J., Hay R. T. 1997; Role of preterminal protein processing in adenovirus replication. J Virol 71:6381–6389
    [Google Scholar]
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