RT Journal Article SR Electronic(1) A1 Mathewson, Alison C. A1 Bishop, Alexandra A1 Yao, Yongxiu A1 Kemp, Fred A1 Ren, Junyuan A1 Chen, Hongying A1 Xu, Xiaodong A1 Berkhout, Ben A1 van der Hoek, Lia A1 Jones, Ian M.YR 2008 T1 Interaction of severe acute respiratory syndrome-coronavirus and NL63 coronavirus spike proteins with angiotensin converting enzyme-2 JF Journal of General Virology, VO 89 IS 11 SP 2741 OP 2745 DO https://doi.org/10.1099/vir.0.2008/003962-0 PB Microbiology Society, SN 1465-2099, AB Although in different groups, the coronaviruses severe acute respiratory syndrome-coronavirus (SARS-CoV) and NL63 use the same receptor, angiotensin converting enzyme (ACE)-2, for entry into the host cell. Despite this common receptor, the consequence of entry is very different; severe respiratory distress in the case of SARS-CoV but frequently only a mild respiratory infection for NL63. Using a wholly recombinant system, we have investigated the ability of each virus receptor-binding protein, spike or S protein, to bind to ACE-2 in solution and on the cell surface. In both assays, we find that the NL63 S protein has a weaker interaction with ACE-2 than the SARS-CoV S protein, particularly in solution binding, but the residues required for contact are similar. We also confirm that the ACE-2-binding site of NL63 S lies between residues 190 and 739. A lower-affinity interaction with ACE-2 might partly explain the different pathological consequences of infection by SARS-CoV and NL63., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.2008/003962-0