Very virulent infectious bursal disease virus: reduced pathogenicity in a rare natural segment-B-reassorted isolate Nouën, Cyril Le and Rivallan, Gaëlle and Toquin, Didier and Darlu, Pierre and Morin, Yannick and Beven, Véronique and de Boisseson, Claire and Cazaban, Christophe and Comte, Sylvain and Gardin, Yannick and Eterradossi, Nicolas,, 87, 209-216 (2006), doi = https://doi.org/10.1099/vir.0.81184-0, publicationName = Microbiology Society, issn = 0022-1317, abstract= The purpose of this study was to compare the molecular epidemiology of infectious bursal disease virus (IBDV) segments A and B of 50 natural or vaccine IBDV strains that were isolated or produced between 1972 and 2002 in 17 countries from four continents, with phenotypes ranging from attenuated to very virulent (vv). These strains were subjected to sequence and phylogenetic analysis based on partial sequences of genome segments A and B. Although there is co-evolution of the two genome segments (70 % of strains kept the same genetic relatives in the segment A- and B-defined consensus trees), several strains (26 %) were identified with the incongruence length difference test as exhibiting a significantly different phylogenetic relationship depending on which segment was analysed. This suggested that natural reassortment could have occurred. One of the possible naturally occurring reassortant strains, which exhibited a segment A related to the vvIBDV cluster whereas its segment B was not, was thoroughly sequenced (coding sequence of both segments) and submitted to a standardized experimental characterization of its acute pathogenicity. This strain induced significantly less mortality than typical vvIBDVs; however, the mechanisms for this reduced pathogenicity remain unknown, as no significant difference in the bursal lesions, post-infectious antibody response or virus production in the bursa was observed in challenged chickens., language=, type=