1887

Abstract

(HAV) is a hepatotropic member of the family . Despite a remarkable antigenic stability, recent results have shown that HAV exists and in cell culture as distributions of genetically related, non-identical variants, referred to as quasispecies. To gain insight into HAV evolution over time in a specific geographical region, genotype I consensus sequences from strains isolated in France in consecutive years were studied. Phylogenetic neighbour-joining method and a non-hierarchical partition analysis, designed to analyse viral quasispecies, indicate that at least five distinct subpopulations of HAV were identified in the course of the disease episode. Strikingly, over time, different subpopulations cycled in dominance. The coexistence of distinct subpopulations whose frequency varies with time is consistent with quasispecies dynamics, and suggests that variation in the dominant HAV population may provide HAV adaptability without being reflected in significant antigenic variation.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.81286-0
2006-01-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/87/1/115.html?itemId=/content/journal/jgv/10.1099/vir.0.81286-0&mimeType=html&fmt=ahah

References

  1. Agol V. A. 2002; Picornavirus genome: an overview. In Molecular Biology of Picornaviruses pp  127–148 Edited by Semler B., Wimmer E. Washington, DC: ASM Press;
    [Google Scholar]
  2. Baccam P., Thompson R. J., Fedrigo O., Carpenter S., Cornette J. L. 2001; PAQ: partition analysis of quasispecies. Bioinformatics 17:16–22 [CrossRef]
    [Google Scholar]
  3. Baccam P., Thompson R. J., Li Y. & 7 other authors 2003; Subpopulations of equine infectious anemia virus Rev coexist in vivo and differ in phenotype. J Virol 77:12122–12131 [CrossRef]
    [Google Scholar]
  4. Costa-Mattioli M., Cristina J., Romero H. & 12 other authors 2002; Molecular evolution of hepatitis A virus: a new classification based on the complete VP1 protein. J Virol 76:9516–9525 [CrossRef]
    [Google Scholar]
  5. Costa-Mattioli M., Ferre V., Casane D. & 7 other authors 2003; Evidence of recombination in natural populations of hepatitis A virus. Virology 311:51–59 [CrossRef]
    [Google Scholar]
  6. Domingo E., Biebricher C., Eigen M., Holland J. J. 2001 Quasispecies and RNA Virus Evolution: Principles and Consequences Austin, TX: Landes Bioscience;
    [Google Scholar]
  7. Felsenstein J. 1993 phylip (phylogeny inference package) version 3.572c. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  8. Kumar S., Tamura K., Nei M. 1994; mega: molecular evolutionary genetics analysis software for microcomputers. Comput Appl Biosci 10:189–191
    [Google Scholar]
  9. Palmenberg A. C. 1989; Sequence alignments of picornaviral capsid proteins. In Molecular Aspects of Picornaviral Infection and Detection Edited by Semler B. L., Ehenfeld E. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  10. Papathanasopoulos M. A., Hunt G. M., Tiemessen C. T. 2003; Evolution and diversity of HIV-1 in Africa – a review. Virus Genes 26:151–163 [CrossRef]
    [Google Scholar]
  11. Robertson B. H., Jansen R. W., Khanna B. & 10 other authors 1992; Genetic relatedness of hepatitis A virus strains recovered from different geographical regions. J Gen Virol 73:1365–1377 [CrossRef]
    [Google Scholar]
  12. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  13. Sanchez G., Bosch A., Gomez-Mariano G., Domingo E., Pinto R. M. 2003a; Evidence for quasispecies distributions in the human hepatitis A virus genome. Virology 315:34–42 [CrossRef]
    [Google Scholar]
  14. Sanchez G., Bosch A., Pinto R. M. 2003b; Genome variability and capsid structural constraints of hepatitis A virus. J Virol 77:452–459 [CrossRef]
    [Google Scholar]
  15. Shapshak P., Segan D. M., Crandall K. A. & 7 other authors 1999; Independent evolution of HIV type 1 in different brain regions. AIDS Res Hum Retroviruses 15:811–820 [CrossRef]
    [Google Scholar]
  16. Sneath P. H. A., Sokal R. R. 1973 Numerical Taxonomy San Francisco: Freeman;
    [Google Scholar]
  17. Swofford D. L. 1999 paup*. Phylogenetic Analysis Using Parsimony (and Other methods), version 4 Sunderland, MA: Sinauer Associates;
    [Google Scholar]
  18. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  19. Ticehurst J., Cohen J. I., Feinstone S. M., Purcell R. H., Jansen R. W., Lemon S. M. 1989; Replication of hepatitis A virus: new ideas from studies with cloned cDNA. In Molecular Aspects of Picornaviral Infection and Detection pp  27–50 Edited by Semler B. L., Ehenfeld E. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  20. Van Regenmortel M. H. V. 2000 In Virus Taxonomy. Seventh Report of the International Committee on Taxonomy of Viruses Edited by van Regenmortel M. H. V., Fauquet C. M., Bishop D. H. L., Carstens E. B., Estes M. K., Lemon S. M., Maniloff J., Mayo M. A., McGeoch D. J., Pringle C. R., Wickner R. B. San Diego: Academic Press;
    [Google Scholar]
  21. Wimmer E., Murdin A. 1991; Hepatitis A and the molecular biology of picornaviruses: a case for a new genus of the family Picornaviridae . In Viral Hepatitis and Liver Disease pp  1–41 Edited by Hollinger F. B., Lemon S. M., Margolis H. S. Baltimore: Williams & Wilkins;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.81286-0
Loading
/content/journal/jgv/10.1099/vir.0.81286-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error