Infectious clone construction of dengue virus type 2, strain Jamaican 1409, and characterization of a conditional E6 mutation
Pierro, Dennis J. and Salazar, Ma Isabel and Beaty, Barry J. and Olson, Ken E.,, 87, 2263-2268 (2006),
doi = https://doi.org/10.1099/vir.0.81958-0,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= A full-length infectious cDNA clone (ic) was constructed from the genome of the dengue virus type 2 (DENV-2) Jamaica83 1409 strain, pBAC1409ic, by using a bacterial artifical chromosome plasmid system. Infectious virus was generated and characterized for growth in cell culture and for infection in Aedes aegypti mosquitoes. During construction, an isoleucine to methionine (Ile→Met) change was found at position 6 in the envelope glycoprotein sequence between low- and high-passage DENV-2 1409 strains. In vitro-transcribed genomic RNA of 1409ic with E6-Ile produced infectious virions following electroporation in mosquito cells, but not mammalian cells, while 1409ic RNA with an E6-Met mutation produced virus in both cell types. Moreover, DENV-2 1409 with the E6-Ile residue produced syncytia in C6/36 cell culture, whereas viruses with E6-Met did not. However, in vitro cell culture-derived growth-curve data and in vivo mosquito-infection rates revealed that none of the analysed DENV-2 strains differed from each other.,
language=,
type=