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Abstract
Paramyxoviruses are a group of single-stranded, negative-sense RNA viruses, some of which are responsible for acute human disease, including parainfluenza virus, measles virus, Nipah virus and Hendra virus. In recent years, a large number of novel paramyxoviruses, particularly members of the genus Jeilongvirus, have been discovered in wild mammals, suggesting that the diversity of paramyxoviruses may be underestimated. Here we used hemi-nested reverse transcription PCR to obtain 190 paramyxovirus sequences from 969 small mammals in Hubei Province, Central China. These newly identified paramyxoviruses were classified into four clades: genera Jeilongvirus, Morbillivirus, Henipavirus and Narmovirus, with most of them belonging to the genus Jeilongvirus. Using Illumina sequencing and Sanger sequencing, we successfully recovered six near-full-length genomes with different genomic organizations, revealing the more complex genome content of paramyxoviruses. Co-divergence analysis of jeilongviruses and their known hosts indicates that host-switching occurred more frequently in the evolutionary histories of the genus Jeilongvirus. Together, our findings demonstrate the high prevalence of paramyxoviruses in small mammals, especially jeilongviruses, and highlight the diversity of paramyxoviruses and their genome content, as well as the evolution of jeilongviruses.
- Received:
- Accepted:
- Published Online:
Funding
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the Translational Medicine and Interdisciplinary Research Joint Fund of Zhongnan Hospital of Wuhan University
(Award ZNLH202203)
- Principle Award Recipient: NotApplicable
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the Translational Medicine and Interdisciplinary Research Joint Fund of Zhongnan Hospital of Wuhan University
(Award ZNLH201905)
- Principle Award Recipient: NotApplicable
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the Young Elite Scientist Sponsorship Program by CAST
(Award YESS20200394)
- Principle Award Recipient: Liang-junChen
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National Key Research and Development Program of China
(Award 2018YFE0204500)
- Principle Award Recipient: Yi-rongLi
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National Natural Science Foundation of China
(Award 82000021)
- Principle Award Recipient: Liang-junChen
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National Natural Science Foundation of China
(Award 81991220308)
- Principle Award Recipient: WeiHou
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National Natural Science Foundation of China
(Award U20A20396)
- Principle Award Recipient: WeiHou
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National Key R&D Program of China
(Award 2023YFC2605504)
- Principle Award Recipient: NotApplicable