@article{mbs:/content/journal/micro/10.1099/00221287-134-9-2475, author = "Dickinson, J. Richard and Smith, Maxine E. and Swanson, Timothy R. and Williams, Anthony S. and Wingfield, Jonathan M.", title = "The cdc30 Mutation in Saccharomyces cerevisiae Affects Phosphoglucose Isomerase, the Cell Cycle and Sporulation", journal= "Microbiology", year = "1988", volume = "134", number = "9", pages = "2475-2480", doi = "https://doi.org/10.1099/00221287-134-9-2475", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-134-9-2475", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Spontaneous revertants of the cdc30 mutation in Saccharomyces cerevisiae simultaneously regained the ability to grow and divide at 36·5 °C on glucose-containing media along with a more thermostable phosphoglucose isomerase (PGI). An independently isolated allele of cdc30 gave a similar phenotype to that previously described including temperature-sensitivity of PGI. Isoelectric focussing allowed the separation of two isoenzymes of PGI. These results all support the idea that two genes - PGI1 and CDC30 - are responsible for PGI activity in yeast. Diploid strains homozygous for the cdc30 mutation sporulated poorly in potassium acetate irrespective of whether the cells had previously been cultured at a temperature that was permissive or restrictive for cell cycle progression. This was not surprising because a strain defective in PGI would not be expected to be able to complete the gluconeogenic events of sporulation.", }