1887

Abstract

cells have bipolar flagella. Both flagella have similar lengths of about one helical turn, or 3.53±0.52 µm. The flagellar filament is composed of two homologous flagellins: FlaA and FlaB. Mutant strains that express either FlaA or FlaB alone produce filaments that are shorter than those of the wild-type. It is reported that the gene could affect filament length in some species of bacteria, but its function remains unknown. We introduced a -deletion mutation into the wild-type strain and - or -deletion mutant strains, and observed their flagella by microscopy. The Δ mutant cells produced long filaments of two helical turns in the wild-type background. The Δ double mutant cells produced very short FlaB filaments. On the other hand, Δ double mutant cells produced long FlaA filaments and their morphology was not helical but straight. Furthermore, FlaG was secreted, and a pulldown assay showed that sigma factor 28 was co-precipitated with purified polyhistidine-tagged FlaG. We conclude that FlaG controls flagella length by negatively regulating FlaA filament assembly and discuss the role of FlaA and FlaB flagellins in flagella formation.

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2018-05-01
2024-03-28
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