1887

Abstract

Summary

Earlier work suggested that the antiviral activity in extracts from lesions of molluscum contagiosum was due to the molluscum virus present. This has now been supported by showing a direct relationship between inhibitory activity and electron microscopically determined virus particle count in extracts from 14 patients. Using this antiviral activity as a biological marker, an analysis of the adsorption of the molluscum virus to mouse embryo cell monolayers was made. In general, the pattern of adsorption of the molluscum agent, with respect to time, temperature and inoculum volume, was similar to that already recorded in the literature for adsorption to cell cultures of the infectivity of vaccinia virus. There was a short delay between adsorption of molluscum virus and the onset of challenge virus plaque inhibition. There-after inhibition increased progressively for several hours.

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/content/journal/jgv/10.1099/0022-1317-1-3-269
1967-07-01
2024-05-04
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References

  1. Allison A. C., Valentine R. C. 1960; Virus particle adsorption. III. Adsorption of viruses by cell monolayers and effects of some variables on adsorption. Biochim. biophys. Acta 40:400
    [Google Scholar]
  2. Bradley D. E. 1961; Techniques for mounting, dispersing and disintegrating specimens. In Techniques for Electron Microscopy, 1st ed. p. 66 Kay Desmond. Ed. by Oxford: Blackwell;
    [Google Scholar]
  3. Dulbecco R., Vogt M. 1954; Plaque formation and isolation of pure lines with poliomyelitis viruses. J. exp. Med 99:167
    [Google Scholar]
  4. Harris W. J., Westwood J. C. N. 1964; Phosphotungstate staining of vaccinia virus. J. gen. Microbiol 34:491
    [Google Scholar]
  5. Neva F. A. 1962; Studies on molluscum contagiosum. Observations on the cytopathic effect of molluscum suspensions in vitro. Archs intern. Med 110:720
    [Google Scholar]
  6. Postlethwaite R. 1960; A plaque technique for the titration of vaccinia virus in chick embryo cells and some features of vaccinial infection in this system. Virology 10:466
    [Google Scholar]
  7. Postlethwaite R. 1964; Antiviral activity in extracts from lesions of molluscum contagiosum. Virology 22:508
    [Google Scholar]
  8. Watson D. H. 1962; Particle counts on herpes virus in phosphotungstate negatively stained preparations. In Fifth Int. Cong. Electron Microsc Breese S. S.Jun Ed. by 2 p. x-4 New York and London: Academic Press;
    [Google Scholar]
  9. Williams M. G., Almeida J. D., Howatson A. F. 1962; Electron microscope studies on viral skin lesions. A simple and rapid method of identifying virus particles. Archs Derm 86:290
    [Google Scholar]
  10. Youngner J. S. 1956; Virus adsorption and plaque formation in monolayer cultures of trypsin-dispersed monkey kidney. J. Immun 76:288
    [Google Scholar]
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