f Characteristics of a Human Cell Line Transformed by DNA from Human Adenovirus Type 5
- Authors: F. L. Graham*, J. Smiley†, W. C. Russell, R. Nairn‡
- First Published Online: 01 July 1977, Journal of General Virology 36: 59-72, doi: 10.1099/0022-1317-36-1-59
- Subject: Articles
- Published Online:
Human embryonic kidney cells have been transformed by exposing cells to sheared fragments of adenovirus type 5 DNA. The transformed cells (designated 293 cells) exhibited many of the characteristics of transformation including the elaboration of a virus-specific tumour antigen. Analysis of the polypeptides synthesized in the 293 cells by labelling with 35S-methionine and SDS PAGE showed a variable pattern of synthesis, different in a number of respects from that seen in other human cells. On labelling the surface of cells by lactoperoxidase catalysed radio-iodination, the absence of a labelled polypeptide analogous to the 250 K (LETS) glycoprotein was noted. Hybridization of labelled cellular RNA with restriction fragments of adenovirus type 5 DNA indicated transcription of a portion of the adenovirus genome at the conventional left hand end.
Research Student of the National Cancer Institute of Canada.
Present address: Department of Microbiology and Immunology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York, N.Y. 10461, USA.
© Society for General Microbiology 1977 | Published by the Microbiology Society
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